Initiation of DNA synthesis in cell cultures by colcemid.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 5 ·1971-05-00 ·Pages 977-9

Vasiliev JM, Gelfand IM, Guelstein VI

Abstract

Mitotic inhibitors (colcemid, colchicine, and vinblastine) initiate DNA synthesis in dense stationary cultures of mouse embryo fibroblast-like cells. This initiation is not due to any changes in local cell population density. Relationships between the activation of proliferation and other changes of interphase fibroblast-like cells produced by the mitotic inhibitors (disappearance of cytoplasmic microtubules and activation of movements of cell surface) are discussed.

MeSH Terms

Animals Benzocycloheptenes/pharmacology Colchicine/pharmacology Culture Techniques DNA/biosynthesis Embryo, Mammalian/cytology Fibroblasts/drug effects,metabolism Mice Microtubules/drug effects Stimulation, Chemical Thymidine/metabolism Tritium Vinblastine/pharmacology

Chemicals

Benzocycloheptenes Tritium Vinblastine DNA Colchicine Thymidine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Vasiliev J M

Gelfand I M

Guelstein V I

References (14)

14 references, click to expand

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Nature. 1968 Jan 20;217(5125):268-9 PMID: 5639135
Surface changes disturbing intracellular homeostasis as a factor inducing cell growth and division.
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Proc Natl Acad Sci U S A. 1968 May;60(1):300-4 PMID: 5241531
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Nature. 1970 Jul 11;227(5254):170-1 PMID: 5428405
Requirement of serum for DNA synthesis in BHK 21 cells: effects of density, suspension and virus transformation.
Nature. 1970 Aug 22;227(5260):798-801 PMID: 4317353
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Nature. 1970 Aug 22;227(5260):843-5 PMID: 5464581
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.5.977

Published

1971-05-00

Pages

977-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389094

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.5.977 PMC Full Text View journal details

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Initiation of DNA synthesis in cell cultures by colcemid.

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