Rapid and complete purification of acetylcholinesterases of electric eel and erythrocyte by affinity chromatography.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 2 ·1971-02-00 ·Pages 395-8

Berman JD, Young M

Abstract

Affinity chromatography has been used to purify acetylcholinesterase both from the electric tissue of Electrophorus electricus and from bovine erythrocyte membranes. For this purpose, several specific enzymic inhibitors of each protein were synthesized and joined covalently to an insoluble support resin. AchE is selectively retained by such inhibitor-resins when highly impure solutions are chromatographed upon them. After removal from the resin, both enzymes are electrophoretically homogeneous and they may be recovered in yields of 75% or more.

MeSH Terms

Acetylcholinesterase/isolation & purification Animals Cattle Cell Membrane/enzymology Cholinesterase Inhibitors Chromatography

Chemicals

Cholinesterase Inhibitors Acetylcholinesterase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Berman J D

Young M

References (9)

9 references, click to expand

Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.
J Biol Chem. 1970 Jun;245(12):3059-65 PMID: 5432796
Affinity labeling of the acetylcholine-receptor.
Proc Natl Acad Sci U S A. 1967 Nov;58(5):2063-70 PMID: 5237500
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.
Ann N Y Acad Sci. 1964 Dec 28;121:404-27 PMID: 14240539
The preparation and properties of some tritiated diazonium salts and related compounds.
Biochemistry. 1967 Mar;6(3):881-7 PMID: 6025570
Purification of cholinesterase from ox red cells.
Biochim Biophys Acta. 1955 Feb;16(2):300 PMID: 14363274
Purification of human red cell acetylcholinesterase by electrophoresis, ultracentrifugation and gradient extraction.
Arch Biochem Biophys. 1955 Jun;56(2):469-75 PMID: 14377597
Acetylcholinesterase, I. Large-scale purification, homogeneity, and amino Acid analysis.
Proc Natl Acad Sci U S A. 1967 Feb;57(2):446-51 PMID: 16591490
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
Selective enzyme purification by affinity chromatography.
Proc Natl Acad Sci U S A. 1968 Oct;61(2):636-43 PMID: 4971842

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.2.395

Published

1971-02-00

Pages

395-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388946

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.2.395 PMC Full Text View journal details

Analysis Services

Rapid and complete purification of acetylcholinesterases of electric eel and erythrocyte by affinity chromatography.

Contact

WeChat Official Account