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PMID: 5277079 Published · ppublish English Comparative Study Journal Article

Characterization of mitochondrial ribosomes from yeast.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 2 ·1971-02-00 ·Pages 324-8

Morimoto H, Halvorson HO

Abstract

A method for the preparation of mitochondrial ribosomes from yeast is presented. Mitochondrial ribosomes differ from their cytoplasmic counterpart in nucleotide composition, electrophoretic mobility of a number of their respective ribosomal proteins, and thermal denaturation profiles. The mitochondrial and cytoplasmic ribosomes have identical sedimentation coefficients (80 S) and dissociate into 60S and 40S subunits. These findings demonstrate that, in yeast, mitochondrial ribosomes are distinct from cytoplasmic ribosomes.

MeSH Terms
Cytoplasm/ultrastructure Electrophoresis, Polyacrylamide Gel Mitochondria/ultrastructure RNA, Ribosomal Ribosomes/ultrastructure Saccharomyces cerevisiae/ultrastructure Ultracentrifugation
Chemicals
RNA, Ribosomal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Morimoto H
Halvorson H O
References (22)
22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.68.2.324
Published
1971-02-00
Pages
324-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388928
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.68.2.324 PMC Full Text View journal details
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