Photo-affinity labeling of specific acetylcholine-binding sites on membranes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 67 ·No. 4 ·1970-12-00 ·Pages 1688-94

Kiefer H, Lindstrom J, Lennox ES, Singer SJ

Abstract

Acetylcholinesterase of intact red blood cell membranes and the acetylcholine receptor at the neuromuscular junction of whole-frog sartorius muscle have been irreversibly inactivated by photo-affinity labeling with two quaternary ammonium aryl azides. The inactivation requires that the azides, at the time of their photolytic conversion to highly reactive nitrenes, are reversibly bound to the specific acetylcholine-binding sites.

MeSH Terms

Acetylcholine Animals Anura Azides/pharmacology Binding Sites Carbachol/antagonists & inhibitors Cell Membrane/drug effects Cholinesterase Inhibitors Erythrocytes/drug effects Neuromuscular Junction Photochemistry Quaternary Ammonium Compounds/pharmacology Receptors, Drug

Chemicals

Azides Cholinesterase Inhibitors Quaternary Ammonium Compounds Receptors, Drug Carbachol Acetylcholine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Kiefer H

Lindstrom J

Lennox E S

Singer S J

References (14)

14 references, click to expand

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A novel irreversible inhibitor of acetylcholinesterase specifically directed at the anionic binding site: structure-activity relationships.
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Affinity labeling of the anionic site of acetylcholinesterase.
Proc Natl Acad Sci U S A. 1967 Dec;58(6):2296-8 PMID: 5242207
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.67.4.1688

Published

1970-12-00

Pages

1688-94

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC283413

Subset

External Links

PubMed source DOI: 10.1073/pnas.67.4.1688 PMC Full Text View journal details

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Photo-affinity labeling of specific acetylcholine-binding sites on membranes.

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