Peritoneal macrophages as target cells for measuring virus-specific T cell mediated cytotoxicity in vitro.

Journal of immunological methods ·Vol. 8 ·No. 3 ·1975-09-00 ·Pages 263-6

Zinkernagel RM, Doherty PC

Abstract

In inbred or outbred mice lysis of virally modified cells by thymus-derived lymphocytes occurs only within a H-2 compatible system. Peritoneal macrophages provide a convenient source of syngeneic target cells. They are readily infected in vitro with LCM virus, and show high levels of specific 51Cr release when exposed to T cells from LCM immune mice.

MeSH Terms

Animals Antigens, Viral/administration & dosage Ascitic Fluid/immunology Cytotoxicity Tests, Immunologic Epitopes Immunity, Cellular Injections, Intravenous Lymphocytic choriomeningitis virus/immunology Macrophages/immunology Mice Mice, Inbred BALB C Mice, Inbred CBA T-Lymphocytes/immunology

Chemicals

Antigens, Viral Epitopes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Zinkernagel R M

Doherty P C

Article Info

Journal

Journal of immunological methods

Abbr.

J Immunol Methods

ISSN

0022-1759

DOI

10.1016/0022-1759(75)90120-9

Published

1975-09-00

Pages

263-6

Language

English

Region

Netherlands

NLM ID

1305440

Subset

External Links

PubMed source DOI: 10.1016/0022-1759(75)90120-9 View journal details

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Peritoneal macrophages as target cells for measuring virus-specific T cell mediated cytotoxicity in vitro.

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