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PMID: 5264150 Published · ppublish English Journal Article

Effect of the lambda repressor on the binding of RNA polymerase to DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 64 ·No. 3 ·1969-11-00 ·Pages 962-9

Hayward WS, Green MH

Abstract

Experiments were designed with the aim of understanding the mechanism whereby the lambda repressor inhibits messenger RNA synthesis. Complexes containing lambda DNA and RNA polymerase were isolated from repressed and derepressed lysogens and analyzed for the relative amounts of RNA polymerase per phage genome. The data indicate that the repressor inhibits the binding of RNA polymerase to promoter sites known to be under direct control of the repressor.

MeSH Terms
Binding Sites Coliphages/enzymology,metabolism DNA, Viral/metabolism Genes, Regulator Hybridization, Genetic Models, Biological Operon RNA Nucleotidyltransferases/metabolism RNA, Messenger/biosynthesis Rifampin/pharmacology Tritium
Chemicals
DNA, Viral RNA, Messenger Tritium RNA Nucleotidyltransferases Rifampin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hayward W S
Green M H
References (26)
26 references, click to expand
  1. Inhibition of DNA-dependent RNA synthesis by rifamycins.
    J Antibiot (Tokyo). 1968 Mar;21(3):234-6 PMID: 4876998
  2. Specific binding of the lambda phage repressor to lambda DNA.
    Nature. 1967 Apr 15;214(5085):232-4 PMID: 6034235
  3. Asymmetric distribution of the transcribing regions on the complementary strands of coliphage lambda DNA.
    Proc Natl Acad Sci U S A. 1967 Jun;57(6):1618-25 PMID: 5231395
  4. The periodicity of RNA polymerase initiations: a new regulatory feature of transcription.
    Proc Natl Acad Sci U S A. 1968 May;60(1):313-20 PMID: 4872442
  5. The production of phage chromosome fragments and their capacity for genetic transfer.
    J Mol Biol. 1962 Apr;4:275-87 PMID: 14453145
  6. Genetic regulatory mechanisms in the synthesis of proteins.
    J Mol Biol. 1961 Jun;3:318-56 PMID: 13718526
  7. Early-late transcription switch: isolation of a lambda DNA-RNA polymerase complex active in the synthesis of late RNA.
    Biochem Biophys Res Commun. 1968 Jun 28;31(6):919-24 PMID: 4875730
  8. Inactivation of the prophage lambda repressor without induction.
    J Mol Biol. 1966 Mar;16(1):134-48 PMID: 5917727
  9. Orientation and control of transcription in E. coli phage lambda.
    Nature. 1969 Mar 1;221(5183):823-5 PMID: 5765054
  10. Studies of heat-inducible lambda bacteriophage. I. Order of genetic sites and properties of mutant prophages.
    J Mol Biol. 1966 Mar;16(1):149-63 PMID: 5331241
  11. A possible role of C3 gene of lambda prophage in the maintenance of the lysogenic state.
    Virology. 1969 Jun;38(2):358-60 PMID: 4891413
  12. Phage lambda mutants deficient in r-II exclusion.
    Science. 1967 Dec 22;158(3808):1588-9 PMID: 6060364
  13. Regulation of bacteriophage lambda DNA replication.
    Proc Natl Acad Sci U S A. 1967 Dec;58(6):2343-50 PMID: 5242210
  14. Genetic transcription in bacteriophage lambda: studies of lambda m RNA synthesis in vivo.
    J Mol Biol. 1968 Nov 14;37(3):387-406 PMID: 5719216
  15. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.
    J Mol Biol. 1965 Jul;12(3):829-42 PMID: 4955314
  16. Sensitive mutants of bacteriophage lambda.
    Virology. 1961 May;14:22-32 PMID: 13690195
  17. The promoter-operator region of the lac operon of Escherichia coli.
    J Mol Biol. 1968 Dec;38(3):413-20 PMID: 4887877
  18. DNA binding of the lac repressor.
    J Mol Biol. 1968 Jul 14;34(2):365-8 PMID: 4938552
  19. The operators controlled by the lambda phage repressor.
    Proc Natl Acad Sci U S A. 1968 Aug;60(4):1282-7 PMID: 5244737
  20. Recombination between related temperate bacteriophages and the genetic control of immunity and prophage localization.
    Virology. 1957 Dec;4(3):509-21 PMID: 13507311
  21. Action of rifamycins on RNA polymerase.
    Biochim Biophys Acta. 1968 Mar 18;157(1):215-7 PMID: 4171079
  22. The lac operator is DNA.
    Proc Natl Acad Sci U S A. 1967 Dec;58(6):2415-21 PMID: 4873589
  23. SEPARATION OF THE RNA MESSAGE TRANSCRIBED IN RESPONSE TO A SPECIFIC INDUCER.
    Proc Natl Acad Sci U S A. 1963 May;49(5):729-36 PMID: 16591095
  24. Genetics and physiology of defective lysogeny in K12 (lambda): studies of early mutants.
    Virology. 1966 Oct;30(2):224-41 PMID: 5331503
  25. On the initiation of transcription of the tryptophan operon in Escherichia coli.
    Proc Natl Acad Sci U S A. 1968 May;60(1):305-12 PMID: 4872441
  26. The position and orientation of genes in lambda and lambda dg DNA.
    Cold Spring Harb Symp Quant Biol. 1966;31:129-38 PMID: 4866370
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.64.3.962
Published
1969-11-00
Pages
962-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC223329
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.64.3.962 PMC Full Text View journal details
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