A genetic assay for mRNA's of phage T4.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 64 ·No. 1 ·1969-09-00 ·Pages 198-204

Jayaraman R, Goldberg EB

Abstract

A method has been developed by which many gene-specific mRNA's in T4-infected cells can be quantitatively assayed. The method involves separation of complementary strands of phage T4 DNA, hybridization of the strands with RNA, digestion of nonhybridized regions of DNA with an endonuclease specific for single-stranded DNA, and assay of protected genetic markers by transformation. It has been shown that the gene gammaIIB is transcribed early from the light strand and that the gene 21 is transcribed late from the heavy strand.

MeSH Terms

Coliphages/analysis Genetic Code Hybridization, Genetic Methods RNA, Messenger/analysis RNA, Viral/analysis Transformation, Genetic

Chemicals

RNA, Messenger RNA, Viral

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Jayaraman R

Goldberg E B

References (12)

12 references, click to expand

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ON THE TOPOGRAPHY OF THE GENETIC FINE STRUCTURE.
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Proc Natl Acad Sci U S A. 1968 May;60(1):329-36 PMID: 4968634
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.64.1.198

Published

1969-09-00

Pages

198-204

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC286147

Subset

External Links

PubMed source DOI: 10.1073/pnas.64.1.198 PMC Full Text View journal details

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