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PMID: 525819 Published · ppublish English Journal Article

The migration of myogenic cells from the somites into the leg region of avian embryos. An ultrastructural study.

Anatomy and embryology ·Vol. 157 ·No. 3 ·1979-00-00 ·Pages 291-309

Jacob M, Christ B, Jacob HJ

Abstract

The migration of myogenic stem cells into the leg anlagen of chick embryos between stages 16--20 of Hamburger and Hamilton was examined. SEM and TEM studies reveal that cell migration starts at stage 16 from the just-formed somites 26-28. The migrating myogenic cells are elongated and oriented in a medio-lateral direction. The leading ends branch into filopodia which contact a fibrillar network. At first, single cells migrate; later on the cells leaving the ventro-lateral edge of the dermatome migrate in strands and have specialized contacts between them. After reaction with ruthenium red and concanavalin A the migrating cells show a thick surface coat to which ruthenium red-positive particles are attached. The surface coat may be important in the interactions among the migrating cells as well as between the cells and the substrate. The migration of myogenic stem cells was found to take place in a matrix of collagenous fibrils and ruthenium red-positive particles, probably containing glycosaminoglycans. At the onset of migration the fibrillar network exhibits a preferred medio-lateral orientation. Therefore, it may be concluded that this alignment of the fibrils influences the direction of cell migration.

MeSH Terms
Animals Chick Embryo/ultrastructure Leg/embryology Microscopy, Electron, Scanning Muscles/embryology Time Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jacob M
Christ B
Jacob H J
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Article Info
Journal
Anatomy and embryology
Abbr.
Anat Embryol (Berl)
ISSN
0340-2061
DOI
10.1007/BF00304995
Published
1979-00-00
Pages
291-309
Language
English
Region
Germany
NLM ID
7505194
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00304995
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