A muscarone-binding material in electroplax and its relation to the acetylcholine receptor. I. Centrifugal assay.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 63 ·No. 2 ·1969-06-00 ·Pages 496-503

O'Brien RD, Gilmour LP

Abstract

A muscarone-binding material has been found in Torpedo electroplax. The material behaves in several ways as one would expect of the binding component of the acetylcholine receptor, especially in its high affinity for muscarone (approximate K = 7 x 10(-6)M), acetylcholine (approximate K = 1.1 x 10(-6)M), curare, and nicotine, and its insensitivity to noncholinergic agents; the reversibility of its muscarone binding; and its suitably small amount (about 1.8 nmole/gm). The material appears not to be acetylcholinesterase.

MeSH Terms

Acetylcholine Animals Binding Sites Eels Hydrogen-Ion Concentration Parasympathomimetics Receptors, Drug

Chemicals

Parasympathomimetics Receptors, Drug Acetylcholine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

O'Brien R D

Gilmour L P

References (13)

13 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.63.2.496

Published

1969-06-00

Pages

496-503

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC223591

Subset

External Links

PubMed source DOI: 10.1073/pnas.63.2.496 PMC Full Text View journal details

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A muscarone-binding material in electroplax and its relation to the acetylcholine receptor. I. Centrifugal assay.

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