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PMID: 5257136 Published · ppublish English Journal Article

Interaction of insulin with the cell membrane: the primary action of insulin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 63 ·No. 2 ·1969-06-00 ·Pages 450-7

Cuatrecasas P

Abstract

Insulin can be covalently attached to a large polymers of Sepharose through the alpha-amino group of the N-terminal residue of the B chain, or through the epsilon-amino group of its lysyl residue. Such derivatives effectively increase the utilization of glucose, and suppress the hormone-stimulated lipolysis, of isolated fat cells. The effects occur with concentrations of insulin-Sepharose that are nearly as low as those of native insulin, and the maximal responses are the same. The results indicate that interaction of insulin with superficial membrane structures alone may suffice to initiate transport as well as other metabolic alterations.

MeSH Terms
Adipose Tissue/cytology Amino Acids Binding Sites Biological Transport Carbon Isotopes Cell Membrane/drug effects Glucose/metabolism Insulin/pharmacology Polymers
Chemicals
Amino Acids Carbon Isotopes Insulin Polymers Glucose
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Cuatrecasas P
References (36)
36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.63.2.450
Published
1969-06-00
Pages
450-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC223585
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.63.2.450 PMC Full Text View journal details
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