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PMID: 5256226 Published · ppublish English Journal Article

DNA- dependent RNA-directed protein synthesis in vitro. I. Extent of genome transcription.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 62 ·No. 2 ·1969-02-00 ·Pages 483-9

Bryan RN, Sugiura M, Hayashi M

Abstract

We have described an in vitro system which couples UTP incorporation into RNA by DNA-dependent RNA polymerase with RNA-directed amino acid incorporation into polypeptides. Using the technique of DNA-RNA hybridization, we have demonstrated that the whole PhiX-174 genome is transcribed during concomitant protein synthesis.

MeSH Terms
Carbon Isotopes Centrifugation, Density Gradient Coliphages DNA, Viral Genetic Code Hybridization, Genetic In Vitro Techniques Kinetics RNA Nucleotidyltransferases/metabolism RNA, Viral/metabolism Tritium Uracil Nucleotides/metabolism Valine/metabolism Viral Proteins/biosynthesis
Chemicals
Carbon Isotopes DNA, Viral RNA, Viral Uracil Nucleotides Viral Proteins Tritium RNA Nucleotidyltransferases Valine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bryan R N
Sugiura M
Hayashi M
References (18)
18 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.62.2.483
Published
1969-02-00
Pages
483-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC277824
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.62.2.483 PMC Full Text View journal details
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