The nucleotide sequences of some large ribonuclease T 1 products from bacteriophage R17 ribonucleic acid.

The Biochemical journal ·Vol. 124 ·No. 2 ·1971-09-00 ·Pages 357-66

Jeppesen PG

Abstract

A method of ;fingerprinting' high-molecular-weight (32)P-labelled RNA species, using a two-dimensional thin-layer-chromatographic separation of ribonuclease T(1) digestion products, has been applied to RNA from the Escherichia coli bacteriophage R17. The ;fingerprinting' technique, besides giving a unique pattern that can be used as a characterization of the RNA, has made it possible to isolate a number of the larger oligonucleotides and to determine their nucleotide sequences.

MeSH Terms

Autoradiography Base Sequence Chromatography, Thin Layer Coliphages/analysis Hydrogen-Ion Concentration Nucleotides/analysis Phosphorus Isotopes Polynucleotides/isolation & purification RNA, Viral/analysis Ribonucleases

Chemicals

Nucleotides Phosphorus Isotopes Polynucleotides RNA, Viral Ribonucleases

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Jeppesen P G

References (17)

17 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1240357

Published

1971-09-00

Pages

357-66

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1177150

Subset

External Links

PubMed source DOI: 10.1042/bj1240357 PMC Full Text View journal details

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The nucleotide sequences of some large ribonuclease T 1 products from bacteriophage R17 ribonucleic acid.

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