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PMID: 5111877 Published · ppublish English Journal Article

Isolation of germ cell Golgi apparatus from seminiferous tubules of rat testes.

The Journal of cell biology ·Vol. 51 ·No. 1 ·1971-10-00 ·Pages 273-85

Cunningham WP, Mollenhauer HH, Nyquist SE

Abstract

Intact Golgi apparatus have been isolated with good purity from rat testis by a simplified sucrose gradient technique. The procedure is inherently selective in that most of the Golgi apparatus in the isolate are from germ cells in late spermatocyte or early spermatid development. No Sertoli cell Golgi apparatus are present in the fraction. Biochemical analyses showed that the enzyme N-acetylglucosamine galactosyltransferase is enhanced in the band containing Golgi apparatus. Because they are easy to isolate, and because they are involved with the formation of a specific internal cellular component (the acrosome), these Golgi apparatus will be useful objects for comparison with other kinds of Golgi apparatus and for other studies leading to a better understanding of the basic functioning of Golgi apparatus.

MeSH Terms
Animals Cell Fractionation Culture Media Endoplasmic Reticulum Golgi Apparatus/enzymology Male Methods Microscopy, Electron Rats Rats, Inbred Strains Spermatogenesis Spermatozoa Testis/cytology Transferases/analysis Ultracentrifugation
Chemicals
Culture Media Transferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cunningham W P
Mollenhauer H H
Nyquist S E
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27 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.51.1.273
Published
1971-10-00
Pages
273-85
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2108246
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.51.1.273 PMC Full Text View journal details
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