A micro-method for peripheral leucocyte migration in tuberculin sensitivity.

Journal of clinical pathology ·Vol. 24 ·No. 6 ·1971-09-00 ·Pages 533-6

Federlin K, Maini RN, Russell AS, Dumonde DC

Abstract

Inhibition of buffy layer peripheral leucocyte migration by tuberculin purified protein derivative (PPD) from micro-capillaries mounted in small tissue culture chambers correlates in all cases with a positive Mantoux test. This quick and reproducible test of cellular hypersensitivity in man requires only 5-15 ml of blood and is applicable for study in children and in clinical situations where repeated monitoring of cellular immunity may be required.

MeSH Terms

Adolescent Adult Aged Antigens Cell Migration Inhibition Child Child, Preschool Culture Techniques Humans Immunity, Cellular Infant Leukocytes/immunology Methods Microchemistry Middle Aged Tuberculin Test

Chemicals

Antigens

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Federlin K

Maini R N

Russell A S

Dumonde D C

References (10)

10 references, click to expand

Capillary tube migration for detection of human delayed hypersensitivity: difficulties encountered with "buffy coat" cells and tuberculin antigen.
J Immunol. 1969 Aug;103(2):179-84 PMID: 5803782
Interaction of human peripheral lymphocytes and granulocytes in the migration-inhibition reaction.
Acta Med Scand. 1969 Mar;185(3):221-6 PMID: 4980433
Changes in leucocyte migration after renal transplantation.
Br Med J. 1969 Nov 1;4(5678):275-8 PMID: 4899455
Cell-mediated (delayed) hypersensitivity in patients with summer hay-fever.
Lancet. 1969 Dec 13;2(7633):1269-72 PMID: 4187978
Failure to demonstrate leukocyte migration inhibition in human tuberculin hypersensitivity.
Proc Soc Exp Biol Med. 1969 Dec;132(3):928-30 PMID: 5361012
A leucocyte migration technique for in vitro detection of cellular (delayed type) hypersensitivity in man.
Dan Med Bull. 1969 Jan;16(1):1-6 PMID: 4906196
Possible role of Mycoplasma fermentans in pathogenesis of rheumatoid arthritis.
Lancet. 1970 Aug 8;2(7667):277-80 PMID: 4194360
Peripheral leucocyte migration. Inhibited by diphtheroid organisms isolated from patients with rheumatoid arthritis.
Ann Rheum Dis. 1970 Sep;29(5):541-5 PMID: 5529234
'Lymphokines': molecular mediators of cellular immune responses in animals and man.
Proc R Soc Med. 1970 Sep;63(9):899-902 PMID: 5477067
The clinical significance of mediators of cellular immunity.
Clin Allergy. 1971 Mar;1(1):123-39 PMID: 4949099

Article Info

Journal

Journal of clinical pathology

Abbr.

J Clin Pathol

ISSN

0021-9746

DOI

10.1136/jcp.24.6.533

Published

1971-09-00

Pages

533-6

Language

English

Region

England

NLM ID

0376601

PMCID

PMC477088

Subset

External Links

PubMed source DOI: 10.1136/jcp.24.6.533 PMC Full Text View journal details

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A micro-method for peripheral leucocyte migration in tuberculin sensitivity.

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