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PMID: 50851 Published · ppublish English Journal Article

Immunity to murine sarcoma virus induced tumours. IV. Direct cellular cytolysis of 51Cr labelled target cells in vitro and analysis of blocking factors which modulate cytotoxicity.

British journal of cancer ·Vol. 31 ·No. 4 ·1975-04-00 ·Pages 387-404

Gorczynski RM, Knight RA

Abstract

The antigen specific cell mediated cytotoxicity of MSV immune spleen lymphocytes to 51Cr labelled murine lymphoma cells was wholly abolished by pretreatment of the spleen cells with anti-theta antibody and complement. Early during the immune response to MSV the cytotoxic acitivity was inhibited by incubation of immune lymphocytes with "late progressor" or "early regressor" serum. Immune lymphocytes at later times were more refractory to such inhibition by serum blocking factors. Although unfractionated cytotoxic lymphocytes, irrespective of the time after MSV infection at which they were tested, were inhibited by soluble tumour associated antigen (TAA), a subpopulation of cytotoxic T cells was identified which was inhibited neither by antigen nor serum.

MeSH Terms
Animals Antigens, Neoplasm Antigens, Viral Cytotoxicity Tests, Immunologic Epitopes Gammaretrovirus Immunity, Cellular Lymphoma/immunology Mice Mice, Inbred BALB C Mice, Inbred C57BL Neoplasms, Experimental/immunology Sarcoma Viruses, Murine Spleen/immunology T-Lymphocytes/immunology Time Factors
Chemicals
Antigens, Neoplasm Antigens, Viral Epitopes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gorczynski R M
Knight R A
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25 references, click to expand
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Article Info
Journal
British journal of cancer
Abbr.
Br J Cancer
ISSN
0007-0920
DOI
10.1038/bjc.1975.78
Published
1975-04-00
Pages
387-404
Language
English
Region
England
NLM ID
0370635
PMCID
PMC2009458
Subset
IM
External Links
PubMed source DOI: 10.1038/bjc.1975.78 PMC Full Text View journal details
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