Studies on the biosynthesis of sym-homospermidine in sandal (Santalum album L.).

The Biochemical journal ·Vol. 127 ·No. 1 ·1972-03-00 ·Pages 61-7

Kuttan R, Radhakrishnan AN

Abstract

The biosynthesis of the newly isolated polyamine, sym-homospermidine (NH(2)-[CH(2)](4)-NH-[CH(2)](4) -NH(2)), was studied by using radioactive amino acids. Arginine was the most effective precursor, being about 10 times as active as ornithine. Unlabelled agmatine and putrescine markedly inhibited the incorporation of [(14)C]arginine into homospermidine. Similarly the incorporation of ornithine was inhibited by unlabelled arginine and putrescine. gamma-Aminobutyraldehyde, the oxidation product of putrescine, was considered to be one of the intermediates in the biosynthesis of homospermidine. The biosynthesis may involve a Schiff-base formation of putrescine with gamma-aminobutyraldehyde and subsequent reduction. A limited synthesis of spermidine also takes place under these conditions.

MeSH Terms

Aldehydes Amidines/pharmacology Arginine/metabolism,pharmacology Autoradiography Carbon Isotopes Chromatography, Paper Glutamates/metabolism Ornithine/metabolism Oxidation-Reduction Plants/metabolism Polyamines/isolation & purification Putrescine/pharmacology Spermidine/biosynthesis

Chemicals

Aldehydes Amidines Carbon Isotopes Glutamates Polyamines Arginine Ornithine Spermidine Putrescine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kuttan R

Radhakrishnan A N

References (16)

16 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1270061

Published

1972-03-00

Pages

61-7

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1178559

Subset

External Links

PubMed source DOI: 10.1042/bj1270061 PMC Full Text View journal details

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Studies on the biosynthesis of sym-homospermidine in sandal (Santalum album L.).

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