Purification and characterization of crotonase from Clostridium acetobutylicum.

Acetone Amino Acids/analysis Butyrates Catalysis Chemical Precipitation Chromatography, Gel Chromatography, Ion Exchange Clostridium/enzymology Coenzyme A Electrophoresis, Disc Fatty Acids, Essential Guanidines Hot Temperature Hydro-Lyases/analysis,isolation & purification Hydrogen-Ion Concentration Kinetics Macromolecular Substances Molecular Weight Sodium Dodecyl Sulfate Structure-Activity Relationship Ultracentrifugation

Amino Acids Butyrates Fatty Acids, Essential Guanidines Macromolecular Substances Acetone Sodium Dodecyl Sulfate Hydro-Lyases Coenzyme A