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PMID: 5002821 Published · ppublish English Journal Article

Chloroplast RNA populations in dark-grown, light-grown, and greening Euglena gracilis.

Brown RD, Haselkorn R

Abstract

RNA preparations from dark-grown, light-grown, and greening Euglena gracilis have been compared by polyacrylamide gel electrophoresis and by hybridization to Euglena chloroplast DNA. Chloroplast ribosomal RNA is not detected in dark-grown cells; its abundance increases in greening cells over a 72 hr period until the concentration characteristic of light-grown cells is reached. Other RNA species complementary to chloroplast DNA are present in comparable abundance in light-grown, dark-grown, and greening cells.

MeSH Terms
Autoradiography Chloroplasts/analysis Cytoplasm/analysis DNA/analysis Darkness Electrophoresis, Disc Euglena gracilis/analysis,growth & development Light Molecular Weight Nucleic Acid Hybridization Phosphorus Isotopes RNA/analysis RNA, Ribosomal/analysis Spectrophotometry
Chemicals
Phosphorus Isotopes RNA, Ribosomal RNA DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brown R D
Haselkorn R
References (8)
8 references, click to expand
  1. Synthesis and maturation of cytoplasmic ribosomal RNA in Euglena gracilis.
    J Mol Biol. 1971 Aug 14;59(3):491-503 PMID: 5571596
  2. Transcription during bacteriophage T4 development: synthesis and relative stability of early and late RNA.
    J Mol Biol. 1968 Feb 14;31(3):325-48 PMID: 4866329
  3. Transport and Distribution of Auxin during Tropistic Response. II. The Lateral Migration of Auxin in Phototropism of Coleoptiles.
    Plant Physiol. 1964 May;39(3):341-50 PMID: 16655925
  4. [Properties of ribosomic RNA obtained from a white form of Euglena gracilis].
    Biochim Biophys Acta. 1968 Dec 17;169(2):540-2 PMID: 5705491
  5. [Properties of Euglena gracilis ribosomes and ribosomal RNA].
    Biochim Biophys Acta. 1970 Nov 12;224(1):144-54 PMID: 4923220
  6. Chlorophyll Formation and Photosynthetic Competence in Euglena During Light-Induced Chloroplast Development in the Presence of 3, (3,4-dichlorophenyl) 1,1-Dimethyl Urea (DCMU).
    Plant Physiol. 1967 Dec;42(12):1716-25 PMID: 16656710
  7. Separation and characterization of Euglena gracilis chloroplast single-strand DNA.
    Biochim Biophys Acta. 1970 May 21;209(1):16-23 PMID: 5421962
  8. Ribosomal RNA homologies among distantly related organisms.
    Proc Natl Acad Sci U S A. 1970 Feb;65(2):349-56 PMID: 4984236
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
1971-10-00
Pages
2536-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389463
Subset
IM
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