Involvement of sulfhydryl groups in the -galactosidase of Streptococcus lactis.

Journal of bacteriology ·Vol. 108 ·No. 1 ·1971-10-00 ·Pages 599-600

McFeters GA, Sandine WE, Elliker PR

Abstract

Sulfhydryl oxidants and stabilizers caused changes demonstrating the sulfhydryl content of beta-galactosidase for Streptococcus lactis 7962. Ammonium sulfate (0.85 m) rendered the enzyme insensitive to the oxidants. Titrations revealed 11.5 moles of sulfhydryl per mole of enzyme.

MeSH Terms

Ammonium Sulfate Chemical Phenomena Chemistry Chloromercuribenzoates/pharmacology Chromatography, Gel Edetic Acid/pharmacology Galactosidases/analysis,antagonists & inhibitors,metabolism Spectrophotometry Streptococcus/enzymology Sulfhydryl Reagents/pharmacology

Chemicals

Chloromercuribenzoates Sulfhydryl Reagents Edetic Acid Galactosidases Ammonium Sulfate

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

McFeters G A

Sandine W E

Elliker P R

References (7)

7 references, click to expand

Purification and properties of Streptococcus lactis beta-galactosidase.
J Bacteriol. 1967 Mar;93(3):914-9 PMID: 6025431
Carbohydrate transport in Staphylococcus aureus. V. The accumulation of phosphorylated carbohydrate derivatives, and evidence for a new enzyme-splitting lactose phosphate.
Proc Natl Acad Sci U S A. 1967 Jul;58(1):274-9 PMID: 4292101
Some factors affecting association-dissociation of beta-galactosidase from Streptococcus lactis 7962.
Can J Microbiol. 1969 Jan;15(1):105-10 PMID: 5765166
Comparative study of isoenzyme formation of bacterial beta-galactosidase.
J Bacteriol. 1970 Apr;102(1):79-84 PMID: 4908684
Mechanisms of lactose utilization by lactic acid streptococci: enzymatic and genetic analyses.
J Bacteriol. 1970 Jun;102(3):804-9 PMID: 5429725
Studies on lactic dehydrogenase of heart. III. Action of inhibitors.
J Biol Chem. 1954 May;208(1):225-30 PMID: 13174530
BETA-GALACTOSIDASE OF STREPTOCOCCUS LACTIS.
J Bacteriol. 1965 Apr;89:937-42 PMID: 14276118

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.108.1.599-600.1971

Published

1971-10-00

Pages

599-600

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC247107

Subset

External Links

PubMed source DOI: 10.1128/jb.108.1.599-600.1971 PMC Full Text View journal details

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Involvement of sulfhydryl groups in the -galactosidase of Streptococcus lactis.

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