The effect of protein synthesis inhibition on the entry of messenger RNA into the cytoplasm of sea urchin embryos.

The Journal of cell biology ·Vol. 49 ·No. 3 ·1971-06-00 ·Pages 692-701

Hogan B, Gross PR

Abstract

Emetine is a potent inhibitor of protein synthesis in sea urchin embryos. At a concentration of the drug that rapidly inhibits protein synthesis in blastulae by 95%, uridine incorporation into RNA continues for more than 1 hr and presumptive histone messenger RNA is synthesized and transported into the cytoplasm where it is apparently associated with polyribosomes. Possible explanations of this result and its implications for the "informasome" theory of messenger transport in embryonic cells are discussed.

MeSH Terms

Amino Acids Animals Biological Transport Carbon Isotopes Centrifugation, Density Gradient Cytoplasm/analysis,metabolism DNA/biosynthesis Echinodermata Edetic Acid Electrophoresis, Disc Embryo, Nonmammalian/cytology,metabolism Emetine/pharmacology Histocytochemistry Histones/analysis Morphogenesis/drug effects Protein Biosynthesis Proteins/antagonists & inhibitors RNA/analysis,biosynthesis RNA, Messenger/biosynthesis,metabolism RNA, Ribosomal/biosynthesis Ribosomes/analysis,metabolism Tritium Uridine/metabolism

Chemicals

Amino Acids Carbon Isotopes Histones Proteins RNA, Messenger RNA, Ribosomal Tritium RNA DNA Edetic Acid Uridine Emetine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Hogan B

Gross P R

References (23)

23 references, click to expand

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Article Info

Journal

The Journal of cell biology

Abbr.

J Cell Biol

ISSN

0021-9525

DOI

10.1083/jcb.49.3.692

Published

1971-06-00

Pages

692-701

Language

English

Region

United States

NLM ID

0375356

PMCID

PMC2108483

Subset

External Links

PubMed source DOI: 10.1083/jcb.49.3.692 PMC Full Text View journal details

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The effect of protein synthesis inhibition on the entry of messenger RNA into the cytoplasm of sea urchin embryos.

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