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PMID: 4995821 Published · ppublish English Journal Article

Fate of transforming DNA after uptake by competent Bacillus subtilis: failure of donor DNA to replicate in a recombination-deficient recipient.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 5 ·1971-05-00 ·Pages 1070-4

Davidoff-Abelson R, Dubnau D

Abstract

The fate of radioactively-labeled transforming DNA was studied in a recombination-deficient strain of Bacillus subtilis that carried the recB2 mutation (Rec(-)) and was sensitive to radiation. Experiments performed with extracts of this strain after transformation showed that the recovery of donor transforming activity and the appearance of recombinant transforming activity occurred to the same extent as in the Rec(+) strain. Sucrose gradient analyses revealed that donor-recipient complex is also formed to the same extent in the Rec(-) and Rec(+) strains, but that 80-90% of the donor genetic material in the complex failed to replicate in the Rec(-) mutant.

MeSH Terms
Bacillus subtilis/metabolism Carbon Isotopes Centrifugation, Density Gradient DNA Replication DNA, Bacterial/analysis,metabolism Mutation Recombination, Genetic Transformation, Genetic Trichloroacetic Acid Tritium
Chemicals
Carbon Isotopes DNA, Bacterial Tritium Trichloroacetic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Davidoff-Abelson R
Dubnau D
References (24)
24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.68.5.1070
Published
1971-05-00
Pages
1070-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389115
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.68.5.1070 PMC Full Text View journal details
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