Home LiteratureArticle Details
PMID: 4995820 Published · ppublish English Journal Article

Detection of Australia antigen by radioimmunoassay.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 5 ·1971-05-00 ·Pages 1056-60

Aach RD, Grisham JW, Parker CW

Abstract

A double-antibody radioimmunoassay has been developed for the detection of Australia antigen and antibodies to Australia antigen in human sera. The assay is simple to perform, and can be completed in 18 hr. Based on parallel studies of human sera, it is approximately 2000 times more sensitive than agar gel diffusion and 200 times more sensitive than quantitative complement fixation. Australia antigen or antibody to Australia antigen was demonstrated in 22 of 23 single serum samples obtained from patients acutely ill with the long-incubation form of hepatitis; eight of the sera were negative when evaluated by less sensitive techniques.

MeSH Terms
Animals Complement Fixation Tests Guinea Pigs Hemophilia A/blood Hepatitis B/blood,microbiology Hepatitis B Antigens/analysis Hepatitis B virus/analysis Humans Immunodiffusion Iodine Radioisotopes Liver Diseases/blood Rabbits Radioimmunoassay
Chemicals
Hepatitis B Antigens Iodine Radioisotopes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Aach R D
Grisham J W
Parker C W
References (20)
20 references, click to expand
  1. Particles associated with Australia antigen in the sera of patients with leukaemia, Down's Syndrome and hepatitis.
    Nature. 1968 Jun 15;218(5146):1057-9 PMID: 4231935
  2. A complement-fixation test for measuring Australia antigen and antibody.
    J Infect Dis. 1969 Sep;120(3):383-6 PMID: 4980772
  3. An antigen detected in the blood during the incubation period of serum hepatitis.
    Proc Natl Acad Sci U S A. 1968 Jul;60(3):814-21 PMID: 4970112
  4. VARIATIONS IN AFFINITIES OF ANTIBODIES DURING THE IMMUNE RESPONSE.
    Biochemistry. 1964 Jul;3:996-1008 PMID: 14214095
  5. Hepatitis antigen. Correlation with disease and infectivity of blood-donors.
    Lancet. 1969 May 31;1(7605):1055-9 PMID: 4181727
  6. Studies on rabbit lymphocytes in vitro. XI. Superaddition of anti-allotypic lymphocyte transformation: evidence for multipotent lymphoid cells.
    J Immunol. 1970 Jan;104(1):103-13 PMID: 5411500
  7. Serum hepatitis antigen (SH): rapid detection by high voltage immunoelectroosmophoresis.
    Science. 1970 Aug 7;169(3945):593-5 PMID: 4987681
  8. Immunoassay of insulin with insulin-antibody precipitate.
    Biochem J. 1963 Jul;88:137-46 PMID: 13952078
  9. Preparation of iodine-131 labelled human growth hormone of high specific activity.
    Nature. 1962 May 5;194:495-6 PMID: 14450081
  10. Hemagglutination assay for antigen and antibody associated with viral hepatitis.
    Science. 1970 Oct 16;170(3955):332-3 PMID: 4990052
  11. Rapid screening test for detecting hepatitis-associated antigen.
    Science. 1970 Jul 17;169(3942):298-9 PMID: 4988897
  12. Australia antigen and hepatitis.
    N Engl J Med. 1970 Aug 13;283(7):349-54 PMID: 4246769
  13. Virus-like antigen, antibody, and antigen-antibody complexes in hepatitis measured by complement fixation.
    Science. 1969 Jul 18;165(3890):304-6 PMID: 4306916
  14. Viral hepatitis.
    Bull N Y Acad Med. 1955 May;31(5):341-58 PMID: 14364082
  15. Bacteriophage neutralizing activity of fragments derived from rabbit immunoglobulin by papain digestion.
    J Immunol. 1967 Jan;98(1):94-101 PMID: 4163641
  16. Transmission of serum hepatitis.
    JAMA. 1970 Mar 2;211(9):1509-12 PMID: 4983942
  17. Hydrophobic binding sites on immunoglobulins.
    Biochemistry. 1970 Mar 3;9(5):1074-82 PMID: 4190691
  18. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  19. Some antigenic and physical properties of virus-like particles in sera of hepatitis patients.
    J Immunol. 1969 Jun;102(6):1529-32 PMID: 5785352
  20. Detection of Australia antigen and antibody by means of radioimmunoassay techniques.
    J Infect Dis. 1970 May;121(5):550-4 PMID: 5420319
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.68.5.1056
Published
1971-05-00
Pages
1056-60
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389112
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.68.5.1056 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2