Abstract
Cell-free extracts with high nitrogenase activity were prepared by sonic oscillation and French press treatment from the blue-gree alga Anabaena cylindrica. Extracts were prepared from cells grown on a 95% N(2)-5% CO(2) gas mixture followed by a period of nitrogen starvation under an atmosphere of 95% argon-5% CO(2). No increase in the specific activity of extracts was achieved by breaking heterocysts. Activity (assayed by acetylene reduction) was found to be dependent on adenosine triphosphate (ATP), an ATP-generating system, and a low-potential reductant. Na(2)S(2)O(2) employed as reductant supports higher rates of nitrogenase activity than reduced ferredoxin. The activity is associated with a small-particle fraction that can be sedimented by ultracentrifugation. In contrast to the particulate nitrogenase of Azotobacter, which is stable in air, the A. cylindrica nitrogenase is an oxygen sensitive as nitrogenase prepared from anaerobic bacteria.
MeSH Terms
Acetylene/metabolism
Adenosine Triphosphate/metabolism
Argon
Carbon Dioxide
Cell-Free System
Chromatography, Gas
Culture Media
Cyanobacteria/enzymology,growth & development,metabolism
Electron Transport
Ethylenes/biosynthesis
Nitrogen
Nitrogen Fixation
Oxidation-Reduction
Oxidoreductases/metabolism
Pressure
Sodium
Sulfates
Ultracentrifugation
Vibration
Chemicals
Culture Media
Ethylenes
Sulfates
Carbon Dioxide
Argon
Adenosine Triphosphate
Sodium
Oxidoreductases
Nitrogen
Acetylene
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Smith R V
Evans M C
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