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PMID: 499394 Published · ppublish English Journal Article

Dendritic spikes in Purkinje cells of the guinea pig cerebellum studied in vitro.

Experimental brain research ·Vol. 37 ·No. 2 ·1979-10-00 ·Pages 387-98

Hounsgaard J, Yamamoto C

Abstract

Extracellular spikes were recorded simultaneously from dendrites and somata of Purkinje cells in thin cerebellar sections. Spontaneously occurring dendritic spikes were biphasic with the initial phase positive. Triphasic dendritic spikes with a large negative phase appeared during electrophoretic application of glutamate. In media containing procaine, tetrodotoxin, or high concentrations of KCl, negative dendritic spikes occurred whereas soma spikes were abolished. The negative dendritic spikes were suppressed by CoCl2 or MnCl2. Electrical stimulation elicited climbing fibre responses in somata and large negative waves in dendrites. Spikes of dendritic origin were different from those reflecting electrotonic spread of soma spikes. The relation between soma spikes and active dendritic spikes is discussed.

MeSH Terms
Animals Calcium/pharmacology Culture Techniques Dendrites/drug effects,physiology Electric Stimulation Evoked Potentials/drug effects Glutamates/pharmacology Guinea Pigs Nerve Fibers/physiology Neurons/physiology Potassium Chloride/pharmacology Procaine/pharmacology Purkinje Cells/drug effects,physiology Tetrodotoxin/pharmacology gamma-Aminobutyric Acid/pharmacology
Chemicals
Glutamates Tetrodotoxin Procaine gamma-Aminobutyric Acid Potassium Chloride Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hounsgaard J
Yamamoto C
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22 references, click to expand
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Article Info
Journal
Experimental brain research
Abbr.
Exp Brain Res
ISSN
0014-4819
DOI
10.1007/BF00237721
Published
1979-10-00
Pages
387-98
Language
English
Region
Germany
NLM ID
0043312
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00237721 View journal details
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