Mode of action of streptozotocin.

Journal of bacteriology ·Vol. 105 ·No. 2 ·1971-02-00 ·Pages 580-8

Reusser F

Abstract

Streptozotocin induces rapid degradation of deoxyribonucleic acid (DNA) in actively dividing or resting Bacillus subtilis cells. Difference spectroscopy showed that the antibiotic interacts specifically with cytosine containing mononucleotides in vitro. This interference occurs only within the very narrow pH range of 5 to 5.5 and is reversed immediately upon lowering or increasing the pH. No measurable interaction was observed between isolated DNA and streptozotocin. The possibility is discussed that interaction of streptozotocin with cytosine residues in cellular DNA, although possibly taking place at a very low frequency, may constitute the primary step inducing DNA strand breakage.

MeSH Terms

Anti-Bacterial Agents/pharmacology Aspartic Acid/metabolism Bacillus subtilis/drug effects,growth & development,metabolism Bacterial Proteins/biosynthesis Carbon Isotopes Cell-Free System Centrifugation, Density Gradient Chemical Phenomena Chemistry Culture Media Cytosine Nucleotides DNA/metabolism DNA, Bacterial/biosynthesis,metabolism

Chemicals

Anti-Bacterial Agents Bacterial Proteins Carbon Isotopes Culture Media Cytosine Nucleotides DNA, Bacterial Aspartic Acid DNA

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Reusser F

References (15)

15 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.105.2.580-588.1971

Published

1971-02-00

Pages

580-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC248427

Subset

External Links

PubMed source DOI: 10.1128/jb.105.2.580-588.1971 PMC Full Text View journal details

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Mode of action of streptozotocin.

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