Heterogeneous circular DNA elements in vegetative cultures of Bacillus megaterium.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 64 ·No. 2 ·1969-10-00 ·Pages 592-9

Carlton BC, Helinski DR

Abstract

DNA preparations from two strains of Bacillus megaterium, one which produces megacin and another that is megacin sensitive, were analyzed by dye-buoyant density centrifugation, cesium chloride gradient equilibrium centrifugation in the absence of dye, zone sedimentation in sucrose, and electron microscopy. Both strains were found to contain a substantial proportion of their total DNA as circular, covalently closed, double helical molecules. These DNA elements were heterogeneous in size and appeared slightly less dense than chromosomal DNA. The physiological role and origin of these elements is unknown at present.

MeSH Terms

Bacillus megaterium Centrifugation, Density Gradient Culture Media DNA, Bacterial/analysis Microscopy, Electron Thymidine/metabolism Tritium

Chemicals

Culture Media DNA, Bacterial Tritium Thymidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Carlton B C

Helinski D R

References (16)

16 references, click to expand

A minute circular DNA from Escherichia coli 15.
Proc Natl Acad Sci U S A. 1968 Jul;60(3):992-9 PMID: 4875809
Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.
J Mol Biol. 1962 Jun;4:430-43 PMID: 14498379
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
Biochem J. 1956 Feb;62(2):315-23 PMID: 13293190
Circular DNA forms of colicinogenic factors E1, E2 and E3 from Escherichia coli.
J Mol Biol. 1968 Sep 14;36(2):185-94 PMID: 4939624
Characterization of multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis.
Biochemistry. 1968 Oct;7(10):3513-20 PMID: 4878700
Evidence for circular DNA forms of a bacterial plasmid.
Proc Natl Acad Sci U S A. 1967 Aug;58(2):650-7 PMID: 5233464
A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.
Proc Natl Acad Sci U S A. 1967 May;57(5):1514-21 PMID: 5231757
Multiple-length rings in preparations of phi-X174 replicative form.
Proc Natl Acad Sci U S A. 1967 Oct;58(4):1676-83 PMID: 5237895
Catenated circular DNA molecules in HeLa cell mitochondria.
Nature. 1967 Nov 18;216(5116):647-52 PMID: 6082458
Covalently closed minicircular DNA in Micrococcus lysodeikticus.
Biochem Biophys Res Commun. 1968 Sep 6;32(5):757-62 PMID: 5682298
Circular DNA forms of a bacterial sex factor.
Proc Natl Acad Sci U S A. 1967 Oct;58(4):1731-8 PMID: 5237898
TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.
Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072-8 PMID: 16590310
Generation of higher multiple circular DNA forms in bacteria.
Proc Natl Acad Sci U S A. 1968 Dec;61(4):1406-13 PMID: 4884687
Structure of the replicative form of bacteriphage phi X174. VI. Studies on alkali-denatured double-stranded phi X DNA.
J Mol Biol. 1968 Mar 14;32(2):169-82 PMID: 4868418
EQUILIBRIUM SEDIMENTATION OF MACROMOLECULES IN DENSITY GRADIENTS.
Proc Natl Acad Sci U S A. 1957 Jul 15;43(7):581-8 PMID: 16590059
Circular dimer and catenate forms of mitochondrial DNA in human leukaemic leucocytes.
Nature. 1967 Nov 18;216(5116):652-7 PMID: 6082459

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.64.2.592

Published

1969-10-00

Pages

592-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC223385

Subset

External Links

PubMed source DOI: 10.1073/pnas.64.2.592 PMC Full Text View journal details

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Heterogeneous circular DNA elements in vegetative cultures of Bacillus megaterium.

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