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PMID: 4977446 Published · ppublish English Journal Article

Immunochemical studies of 22S protein from isolated mitotic apparatus.

The Journal of cell biology ·Vol. 41 ·No. 2 ·1969-05-00 ·Pages 577-90

Bibring T, Baxandall J

Abstract

Evidence is presented that the "22S protein" of mitotic apparatus isolated from sea urchin eggs is not microtubule protein. An antibody preparation active against 22S protein is described, and immunochemical studies of the distribution of 22S protein in various cellular fractions and among morphological features of mitotic apparatus are reported. The protein is ubiquitous in the metaphase egg fractions that were tested but is not found in sperm flagella. It is immunologically distinct from proposed microtubule protein isolated from mitotic apparatus by the method of Sakai, and from proposed microtubule protein obtained after extraction with mild acid. It exists in nontubule material of isolated mitotic apparatus but is not detectable in microtubules.

MeSH Terms
Animals Cell Biology Densitometry Echinodermata Electrophoresis, Disc Female Immunochemistry Immunodiffusion Immunoelectrophoresis Immunoglobulin G Male Microscopy, Electron Mitosis Ovum/analysis,cytology Proteins/analysis,isolation & purification Spermatozoa/analysis
Chemicals
Immunoglobulin G Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bibring T
Baxandall J
References (35)
35 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.41.2.577
Published
1969-05-00
Pages
577-90
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2107748
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.41.2.577 PMC Full Text View journal details
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