Determination of amino sugars in mixtures containing glucosamine, galactosamine and muramic acid.

The Biochemical journal ·Vol. 109 ·No. 1 ·1968-08-00 ·Pages 13-8

Stewart-Tull DE

Abstract

A colorimetric method is described whereby the direct quantitative determination of glucosamine, galactosamine and muramic acid can be achieved without previous treatment of the cell-wall hydrolysate, for example by column chromatography. Molar ratios of hexosamines in cell-wall preparations, from a number of bacterial species, determined by this method were found to be in general agreement with previously published results.

MeSH Terms

Amino Sugars/analysis Bacteria/analysis Cell Wall/analysis Chromatography Colorimetry Enterococcus faecalis/cytology Escherichia coli/cytology Galactose Glucosamine/analysis Hexosamines/analysis Micrococcus/cytology Mycobacterium/cytology Proteus/cytology Pseudomonas aeruginosa/cytology Staphylococcus/cytology

Chemicals

Amino Sugars Hexosamines Glucosamine Galactose

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Stewart-Tull D E

References (17)

17 references, click to expand

The sugar composition of streptococcal cell walls and its relation to haemagglutination pattern.
J Gen Microbiol. 1961 Feb;24:253-60 PMID: 13742188
The extraction of cell walls of Pseudomonas aeruginosa with aqueous phenol. The insoluble residue and material from the aqueous layers.
Biochem J. 1967 Nov;105(2):759-65 PMID: 4967075
Chemical structure and biosynthesis of bacterial cell walls.
Bacteriol Rev. 1963 Mar;27:18-55 PMID: 13942447
Identification of amino sugars.
Biochem J. 1959 Jul;72:479-86 PMID: 13813097
[Micromethod for the determination of glucosamine in biological fluids].
Boll Soc Ital Biol Sper. 1952 Apr;28(4):858-9 PMID: 13018525
The cell walls of Pseudomonas aeruginosa. General composition.
Biochem J. 1967 Nov;105(2):749-54 PMID: 4967073
A study of the composition and structure of the cell-wall mucopeptide of micrococcus lysodeikticus.
Biochem J. 1963 Mar;86:468-74 PMID: 14024451
Mycobacterial cell walls. II. Chemical composition of the "basal layer".
J Bacteriol. 1963 Jan;85:24-30 PMID: 13984704
A rapid colorimetric distinction between glucosamine and galactosamine.
Biochim Biophys Acta. 1955 May;17(1):159-60 PMID: 13239653
Inhibition of the biosynthesis of cell-wall mucopeptides by the penicillins. A study of penicillin-sensitive and penicillin-resistant strains of Staphylococcus aureus.
Biochem J. 1961 Dec;81:576-84 PMID: 14493345
Composition of the cell wall of Staphylococcus aureus: its relation to the mechanism of action of penicillin.
J Biol Chem. 1959 Dec;234:3263-8 PMID: 13835291
The determination of glucosamine and galactosamine.
Biochem J. 1955 Dec;61(4):586-9 PMID: 13276341
The determination of amino sugars in the presence of amino acids and glucose.
Biochem J. 1960 Dec;77:508-10 PMID: 13692073
A method for the determination of D-galactosamine in the presence of D-glucosamine.
Biochem J. 1963 Jul;88:132-6 PMID: 14019774
An immunochemical analysis of bacterial mucopeptides.
J Immunol. 1966 Dec;97(6):797-804 PMID: 4959400
A colorimetric method for the determination of glucosamine and chondrosamine.
Biochem J. 1933;27(6):1824-8 PMID: 16745305
The influence of age of culture on the production of adjuvant-active peptidoglycolipids by saprophytic mycobacteria.
Immunology. 1967 Mar;12(3):349-59 PMID: 6021418

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1090013

Published

1968-08-00

Pages

13-8

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1186746

Subset

External Links

PubMed source DOI: 10.1042/bj1090013 PMC Full Text View journal details

Analysis Services

Determination of amino sugars in mixtures containing glucosamine, galactosamine and muramic acid.

Contact

WeChat Official Account