Induced sensitization of normal laboratory animals to Brucella abortus endotoxin.

Journal of bacteriology ·Vol. 95 ·No. 2 ·1968-02-00 ·Pages 286-90

Freedman HH, Fox AE, Willis RS, Schwartz BS

Abstract

A Boivin preparation of Brucella abortus, unlike common enterobacterial endotoxins, failed to depress water intake or increase numbers of hemolysin-producing spleen cells in mice, or to cause delayed inflammatory reactions in rabbit skin. Reactivity to the B. abortus endotoxin was found only in animals which were previously given the endotoxin with, but not necessarily in, complete Freund's adjuvant. Previous treatment with the endotoxin in saline or with only the adjuvant was ineffective. Sensitization appeared within 10 days and waned after 5 weeks. Passive sensitization was obtained with sensitized donor spleen cells but not with serum. Serum antibody titers did not correlate with the appearance and disappearance of sensitization.

MeSH Terms

Animals Antibodies/analysis Brucella abortus/immunology Complement Fixation Tests Endotoxins/pharmacology Female Freund's Adjuvant/pharmacology Hemolysin Proteins/biosynthesis Hypersensitivity, Delayed Mice Skin Tests Spleen/metabolism,transplantation Transplantation, Homologous

Chemicals

Antibodies Endotoxins Hemolysin Proteins Freund's Adjuvant

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Freedman H H

Fox A E

Willis R S

Schwartz B S

References (15)

15 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.95.2.286-290.1968

Published

1968-02-00

Pages

286-90

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC252015

Subset

External Links

PubMed source DOI: 10.1128/jb.95.2.286-290.1968 PMC Full Text View journal details

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Induced sensitization of normal laboratory animals to Brucella abortus endotoxin.

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