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PMID: 4965365 Published · ppublish English Journal Article

Extraction, characterization, and cellular localization of the lipids of Staphylococcus aureus.

Journal of bacteriology ·Vol. 94 ·No. 6 ·1967-12-00 ·Pages 1854-67

White DC, Frerman FE

Abstract

Satisfactory extraction and assay procedures have been developed for the lipids of Staphylococcus aureus. The following lipids have been characterized in detail: the vitamin K(2), which is shown to exist as isoprenologues with side chains of 35, 40, and 45 carbon atoms; monoglucosyldiglyceride and diglucosyldiglyceride, which account for all the carbohydrate in the lipid extracts; the lysyl ester of phosphatidyl glycerol, phosphatidyl glycerol, and cardiolipin, which account for 98% of the phosphate in the lipid extract. The extraction procedure removes 98% of the total bacterial fatty acids. Acidification of the medium before harvest and refluxing in isopropanol are critical in the extraction procedure for the maximal recovery of lysyl-phosphatidyl glycerol and the glucolipids. The lipids have been shown to be a part of the same membrane as the respiratory pigments.

MeSH Terms
Carbonic Anhydrases/metabolism Cell Membrane/analysis Chromatography Chromatography, Paper Chromatography, Thin Layer Cytochromes/metabolism Glucosephosphate Dehydrogenase/metabolism Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism Glycolipids/analysis Lipids/analysis Methods Oxygen Consumption Phosphogluconate Dehydrogenase/metabolism Phospholipids/analysis Quinones/analysis Staphylococcus/analysis,metabolism Vitamin K/analysis
Chemicals
Cytochromes Glycolipids Lipids Phospholipids Quinones Vitamin K Phosphogluconate Dehydrogenase Glucosephosphate Dehydrogenase Glyceraldehyde-3-Phosphate Dehydrogenases Carbonic Anhydrases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
White D C
Frerman F E
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37 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.94.6.1854-1867.1967
Published
1967-12-00
Pages
1854-67
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC276914
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.94.6.1854-1867.1967 PMC Full Text View journal details
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