Studies on human leukocyte motility. I. Effects of alterations in pH, electrolyte concentration, and phagocytosis on leukocyte migration, adhesiveness, and aggregation.

The Journal of experimental medicine ·Vol. 124 ·No. 3 ·1966-09-01 ·Pages 483-99

Bryant RE, DesPrez RM, VanWay MH, Rogers DE

Abstract

Leukocyte migration was studied in a microhematocrit system which permitted evaluation of the separate effects of leukocyte adhesiveness, intrinsic cell motility, and leukocyte clumping. Leukocyte adhesion to glass required the presence of magnesium ion, was totally independent of calcium ion, and was partially dependent on heat-labile plasma factors. Leukocyte migration was unimpaired by marked acidosis or by wide variations in sodium, potassium, or calcium concentrations. Marked impairment of individual leukocyte motility and increased leukocyte aggregation were observed after phagocytosis of particulate matter, suggesting a mechanism facilitating recruitment and retention of leukocytes at areas of microbial invasion.

MeSH Terms

Acidosis Acidosis, Respiratory Arsenicals/pharmacology Calcium/pharmacology Diabetic Ketoacidosis Edetic Acid/pharmacology Humans Hydrogen-Ion Concentration Hyperglycemia Hypoglycemia In Vitro Techniques Leukocytes/cytology Magnesium/pharmacology Neutrophils/cytology Phagocytosis

Chemicals

Arsenicals Edetic Acid Magnesium Calcium

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Bryant R E

DesPrez R M

VanWay M H

Rogers D E

References (10)

10 references, click to expand

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Effect of bacteria and their products on migration of leukocytes.
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STUDIES ON THE EFFECT OF CERTAIN MACROMOLECULAR SUBSTANCES ON THE RESPIRATORY ACTIVITY OF THE LEUCOCYTES OF PERIPHERAL BLOOD.
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Studies of phagocytosis of group A streptococci by polymorphonuclear leucocytes in vitro.
J Exp Med. 1960 Mar 1;111:309-22 PMID: 13714578
Accessory plasma factors involved in the bactericidal test for type-specific antibody to group A Streptococci. II. Human plasma cofactor (s) enhancing opsonization of encapsulated organisms.
J Exp Med. 1963 Jan 1;117:1-17 PMID: 13984363
The effect of tubercle bacilli on the migration of phagocytes in vitro.
Am Rev Tuberc. 1949 May;59(5):562-6 PMID: 18120562
Factors influencing chemotaxis of the polymorphonuclear leucocyte.
Br J Exp Pathol. 1956 Oct;37(5):477-80 PMID: 13374205
Functional and metabolic properties of polymorphonuclear leucocytes. I. Observations on the requirements and consequences of particle ingestion.
J Exp Med. 1960 May 1;111:667-87 PMID: 13694491
THE EFFECT OF TUBERCLE BACILLI ON THE POLYMORPHONUCLEAR LEUCOCYTES OF NORMAL ANIMALS.
J Exp Med. 1950 Mar 31;91(4):381-92 PMID: 19871714

Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.124.3.483

Published

1966-09-01

Pages

483-99

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2138230

Subset

External Links

PubMed source DOI: 10.1084/jem.124.3.483 PMC Full Text View journal details

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Studies on human leukocyte motility. I. Effects of alterations in pH, electrolyte concentration, and phagocytosis on leukocyte migration, adhesiveness, and aggregation.

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