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PMID: 4943274 Published · ppublish English Comparative Study Journal Article

Comparison of two commercially available media for detection of bacteremia.

Applied microbiology ·Vol. 22 ·No. 4 ·1971-10-00 ·Pages 604-7

Washington JA

Abstract

An analysis of 3,795 positive blood cultures obtained from 1,718 patients in a 2.5-year evaluation of Tryptic Soy Broth (TSB) and Thiol Broth is reported. Isolation rates of Actinobacillus and Pseudomonas were significantly greater in TSB, whereas isolation rates of Streptococcus and Corynebacterium (aerobic and anaerobic) were significantly greater in Thiol. Otherwise, the two media were similar. Disregarding contaminants, anaerobic bacteria represented 11% of positive cultures and 20% of patients with bacteremia. Eleven per cent of the patients had polymicrobial bacteremia.

MeSH Terms
Aerobiosis Anaerobiosis Bacteria/classification,isolation & purification Bacteriological Techniques Blood Specimen Collection Culture Media Diagnosis, Differential Humans Sepsis/diagnosis,microbiology Soybeans Species Specificity Sulfhydryl Compounds Trypsin
Chemicals
Culture Media Sulfhydryl Compounds Trypsin
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Washington J A
References (13)
13 references, click to expand
  1. The role of temperature and anticoagulant on the in vitro survival of bacterial in blood.
    J Infect Dis. 1966 Apr;116(2):238-42 PMID: 4379627
  2. Studies on the bacteremia of bacterial endocarditis.
    JAMA. 1967 Oct 16;202(3):199-203 PMID: 4860941
  3. Effect of various anticoagulants and no anticoagulant on ability to isolate bacteria directly from parallel clinical blood specimens.
    Am J Clin Pathol. 1968 Feb;49(2):216-9 PMID: 4867431
  4. Evaluation of polyanethol sulfonate (liquoid) in blood cultures.
    Antimicrob Agents Chemother (Bethesda). 1967;7:692-6 PMID: 5596208
  5. System for inoculation of blood in the laboratory.
    Appl Microbiol. 1968 Dec;16(12):1892-4 PMID: 5726162
  6. New medium for blood cultures.
    Appl Microbiol. 1969 Jan;17(1):68-70 PMID: 4886863
  7. Rapid diagnosis of bacteremia.
    Appl Microbiol. 1969 Sep;18(3):458-63 PMID: 4907008
  8. Comparison of a blood culture system containing liquoid and sucrose with systems containing either reagent alone.
    Appl Microbiol. 1970 Feb;19(2):281-2 PMID: 5437303
  9. Polymicrobial bacteremia.
    Ann Intern Med. 1970 Sep;73(3):387-92 PMID: 4917179
  10. Brain abscess with Corynebacterium hemolyticum: report of a case.
    Am J Clin Pathol. 1971 Aug;56(2):212-5 PMID: 5567728
  11. The comparison of percentages in matched samples.
    Biometrika. 1950 Dec;37(3-4):256-66 PMID: 14801052
  12. A practical blood culture procedure.
    Am J Clin Pathol. 1951 Mar;21(3):290-4 PMID: 14818984
  13. The use of a bile-aesculin medium and of Maxted's technique of Lancefield grouping in the identification of enterococci (group D streptococci).
    J Clin Pathol. 1954 May;7(2):160-3 PMID: 13163202
Article Info
Journal
Applied microbiology
Abbr.
Appl Microbiol
ISSN
0003-6919
DOI
10.1128/am.22.4.604-607.1971
Published
1971-10-00
Pages
604-7
Language
English
Region
United States
NLM ID
7605802
PMCID
PMC376371
Subset
IM
External Links
PubMed source DOI: 10.1128/am.22.4.604-607.1971 PMC Full Text
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