Biochemical characterization of the ctr mutants of Escherichia coli.

Journal of bacteriology ·Vol. 108 ·No. 2 ·1971-11-00 ·Pages 690-4

Morse HG, Penberthy WK, Morse ML

Abstract

A test procedure based on complementation in mixed extracts is described for the assay of heat-stable protein and enzyme I of the phosphoenolpyruvate-dependent phosphotransferase system. The test was used to assay a collection of pleiotropic carbohydrate mutants of Escherichia coli (ctr mutants) and revertants of these mutants. All mutants were found to lack enzyme I of the phosphoenolpyruvate-dependent transferase system. Revertants of these mutants to complete wild phenotype regained enzyme I-forming ability. Reversion to partial wild type was not accompanied by restoration of enzyme I-forming ability.

MeSH Terms

Bacterial Proteins/biosynthesis Cell-Free System Culture Media Escherichia coli/enzymology,growth & development,metabolism Genes, Regulator Genetic Complementation Test Genetics, Microbial Glycosides/metabolism Mutation Nitrophenols/biosynthesis Oxidative Phosphorylation Phenotype Phosphoenolpyruvate Phosphotransferases/metabolism Spectrophotometry

Chemicals

Bacterial Proteins Culture Media Glycosides Nitrophenols Phosphoenolpyruvate Phosphotransferases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Morse H G

Penberthy W K

Morse M L

References (10)

10 references, click to expand

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J Mol Biol. 1968 Feb 28;32(1):59-66 PMID: 4868120
Current linkage map of Escherichia coli.
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The role of a phosphoenolpyruvate-dependent kinase system in beta-glucoside catabolism in Escherichia coli.
Proc Natl Acad Sci U S A. 1968 Mar;59(3):988-95 PMID: 4870648
Carbohydrate Accumulation and Metabolism in Escherichia coli: Characteristics of the Reversions of ctr Mutations.
J Bacteriol. 1970 Dec;104(3):1318-24 PMID: 16559110
The physiological behavior of enzyme I and heat-stable protein mutants of a bacterial phosphotransferase system.
J Biol Chem. 1970 Nov 10;245(21):5870-3 PMID: 4919491
Carbohydrate accumulation and metabolism in Escherichia coli: the close linkage and chromosomal location of ctr mutations.
J Bacteriol. 1969 May;98(2):605-10 PMID: 4891263
The enzymatic lesion of strain MM-6, a pleiotropic carbohydrate-negative mutant of Escherichia coli.
Biochem Biophys Res Commun. 1967 Apr 7;27(1):63-7 PMID: 4862174
An agar medium indicating acid production.
J Bacteriol. 1958 Sep;76(3):270-1 PMID: 13575384
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Effect of pleiotropic carbohydrate mutations (ctr) on tryptophan catabolism.
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.108.2.690-694.1971

Published

1971-11-00

Pages

690-4

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC247127

Subset

External Links

PubMed source DOI: 10.1128/jb.108.2.690-694.1971 PMC Full Text View journal details

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Biochemical characterization of the ctr mutants of Escherichia coli.

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