Crystallization and preliminary structural analysis of dihydrolipoyl transsuccinylase, the core of the 2-oxoglutarate dehydrogenase complex.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 6 ·1971-06-00 ·Pages 1135-7

Derosier DJ, Oliver RM, Reed LJ

Abstract

Dihydrolipoyl transsuccinylase, one of the three enzymes comprising the Escherichia coli 2-oxoglutarate dehydrogenase (EC 1.2.4.2) complex, has been crystallized. Studies by x-ray diffraction and electron microscopy establish that the transsuccinylase has octahedral (432) symmetry, i.e., it consists of 24 subunits that are structurally identical.

MeSH Terms

Crystallization Escherichia coli/enzymology Glutarates/metabolism Microscopy, Electron Oxidoreductases/analysis Photography Pyruvates/metabolism Succinates Transferases/analysis X-Ray Diffraction

Chemicals

Glutarates Pyruvates Succinates Oxidoreductases Transferases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Derosier D J

Oliver R M

Reed L J

References (5)

5 references, click to expand

Alpha-keto acid dehydrogenase complexes. VI. Dissociation and reconstitution of the dihydrolipoyl transacetylase of Escherichia coli.
J Biol Chem. 1967 Mar 10;242(5):889-97 PMID: 5335914
Alpha-keto acid dehydrogenase complexes. VII. Isolation and partial characterization of the polypeptide chains in the dihydrolipoyl transacetylase of Escherichia coli.
J Biol Chem. 1967 Mar 10;242(5):898-901 PMID: 5335915
Micro diffusion cells for the growth of single protein crystals by means of equilibrium dialysis.
Arch Biochem Biophys. 1968 Aug;126(2):564-73 PMID: 5672516
The multienzyme alpha-keto acid dehydrogenase complexes.
Brookhaven Symp Biol. 1968 Jun;21(2):397-412 PMID: 4888137
RESOLUTION AND RECONSTITUTION OF THE ESCHERICHIA COLI ALPHA-KETOGLUTARATE DEHYDROGENASE COMPLEX.
J Biol Chem. 1965 May;240:2268-9 PMID: 14299660

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.6.1135

Published

1971-06-00

Pages

1135-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389136

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.6.1135 PMC Full Text View journal details

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Crystallization and preliminary structural analysis of dihydrolipoyl transsuccinylase, the core of the 2-oxoglutarate dehydrogenase complex.

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