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PMID: 4929858 Published · ppublish English Journal Article

Mutation preventing capsular polysaccharide synthesis in Escherichia coli K-12 and its effect on bacteriophage resistance.

Journal of bacteriology ·Vol. 106 ·No. 2 ·1971-05-00 ·Pages 432-7

Radke KL, Siegel EC

Abstract

A mutant strain of Escherichia coli K-12 was found in which spontaneous mutation to phage T7 resistance occurred at a very low frequency. T7 resistance in the parental strain from which this mutant was derived resulted from a mutation to excess capsular polysaccharide synthesis. The mutation preventing T7 resistance, non-9, inhibited capsule formation when transduced into capsulated strains. The non-9 mutation was cotransducible with his, the gene order in this region being non-9 his Su-1.

MeSH Terms
Adsorption Chromosome Mapping Coliphages Cross Reactions Culture Media Escherichia coli/analysis,drug effects,growth & development,metabolism,radiation effects Genetics, Microbial Guanidines Mutagens Mutation Nitroso Compounds Nucleotides/metabolism Pentoses/analysis Polysaccharides, Bacterial/analysis,biosynthesis Recombination, Genetic Transduction, Genetic Ultraviolet Rays
Chemicals
Culture Media Guanidines Mutagens Nitroso Compounds Nucleotides Pentoses Polysaccharides, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Radke K L
Siegel E C
References (25)
25 references, click to expand
  1. Derepression of phosphomannose isomerase by regulator gene mutations involved in capsular polysaccharide synthesis in Escherichia coli K-12.
    J Bacteriol. 1967 Nov;94(5):1497-501 PMID: 4862194
  2. Current linkage map of Escherichia coli.
    Bacteriol Rev. 1970 Jun;34(2):155-75 PMID: 4918631
  3. Control of uridine diphosphate-glucose dehydrogenase synthesis and uridine diphosphate-glucuronic acid accumulation by a regulator gene mutation in Escherichia coli K-12.
    J Bacteriol. 1970 Mar;101(3):959-64 PMID: 4908789
  4. Bacteriophage-Resistant Mutants in Escherichia Coli.
    Genetics. 1945 Mar;30(2):119-36 PMID: 17247150
  5. NATURE OF ETHYL METHANESULFONATE INDUCED REVERSIONS OF LAC-MUTANTS OF ESCHERICHIA COLI.
    Genetics. 1963 Oct;48:1357-75 PMID: 14071756
  6. Mutator gene of Escherichia coli B.
    J Bacteriol. 1967 Jul;94(1):38-47 PMID: 5338974
  7. Genetic mapping of mutations affecting phosphoglucose isomerase and fructose diphosphatase in Escherichia coli.
    J Bacteriol. 1967 May;93(5):1582-7 PMID: 4290578
  8. RESISTANCE AND CROSS RESISTANCE OF ESCHERICHIA COLI S MUTANTS TO THE RADIOMIMETIC AGENT NITROFURAZONE.
    J Bacteriol. 1963 Jun;85:1208-16 PMID: 14047210
  9. Mapping of the galactose genes of Escherichia coli by transduction with phage P1.
    Virology. 1963 Feb;19:117-26 PMID: 14011089
  10. CHROMOSOMAL ABERRATIONS ASSOCIATED WITH MUTATIONS TO BACTERIOPHAGE RESISTANCE IN ESCHERICHIA COLI.
    J Bacteriol. 1965 Jan;89:28-40 PMID: 14255678
  11. THE CAPSULAR POLYSACCHARIDE OF A MUCOID VARIANT OF E. COLI K 12.
    Proc Natl Acad Sci U S A. 1964 Aug;52:265-71 PMID: 14206589
  12. Exopolysaccharide colanic acid and its occurrence in the Enterobacteriaceae.
    J Bacteriol. 1969 Dec;100(3):1187-93 PMID: 4902806
  13. The rigid layer of the cell wall of Escherichia coli strain B.
    J Gen Microbiol. 1960 Feb;22:158-66 PMID: 13843470
  14. Genetic and biochemical studies on mannose-negative mutants that are deficient in phosphomannose isomerase in Escherichia coli K-12.
    J Bacteriol. 1967 Nov;94(5):1492-6 PMID: 4862193
  15. Bacterial viruses; with particular reference to adsorption/penetration.
    Annu Rev Microbiol. 1958;12:27-48 PMID: 13595600
  16. Induction of capsular polysaccharide synthesis by rho-fluorophenylalanine in Escherichia coli wild type and strains with altered phenylalanyl soluble ribonucleic acid synthetase.
    J Bacteriol. 1967 Feb;93(2):584-91 PMID: 5335965
  17. A LOCUS THAT CONTROLS FILAMENT FORMATION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI K-12.
    Genetics. 1964 Feb;49:237-46 PMID: 14124942
  18. Mapping of suppressor loci in Escherichia coli.
    J Mol Biol. 1965 Nov;14(1):153-66 PMID: 5327649
  19. REGULATORY MECHANISMS FOR SYNTHESIS OF CAPSULAR POLYSACCHARIDE IN MUCOID MUTANTS OF ESCHERICHIA COLI K12.
    Proc Natl Acad Sci U S A. 1964 Feb;51:239-46 PMID: 14124321
  20. Depression of guanosine diphosphate-mannose pyrophosphorylase by mutations in two different regulator genes involved in capsular polysaccharide synthesis in Escherichia coli K-12.
    J Bacteriol. 1970 Mar;101(3):965-72 PMID: 4908790
  21. Chromosomal location of the gene determining uridine diphoglucose formation in Escherichia coli K-12.
    J Bacteriol. 1966 Aug;92(2):518-20 PMID: 5330381
  22. Ultraviolet sensitivity gene of Escherichia coli B.
    J Bacteriol. 1968 May;95(5):1555-9 PMID: 4870274
  23. Protection against lethal effects of ultraviolet light on Escherichia coli by capsular polysaccharide.
    Mutat Res. 1970 Aug;10(2):153-5 PMID: 4920266
  24. Capsular protection against virulent coliphage infection.
    Biotechnol Bioeng. 1970 May;12(3):341-6 PMID: 4920047
  25. ANALYSIS OF A GENE CONTROLLING CELL DIVISION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI.
    J Bacteriol. 1964 Mar;87:720-6 PMID: 14127589
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.106.2.432-437.1971
Published
1971-05-00
Pages
432-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC285113
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.106.2.432-437.1971 PMC Full Text View journal details
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