Gliding motility mutants of Myxococcus xanthus.

Journal of bacteriology ·Vol. 104 ·No. 2 ·1970-11-00 ·Pages 940-7

Burchard RP

Abstract

Two gliding motility mutants of Myxococcus xanthus are described. The semimotile mutant (SM) originated by high-frequency segregation from the motile FB(t) strain. Segregation was enhanced by acridine dye treatment. SM cells glide only when apposed to other cells in a swarm. The nonmotile strain (NM) originated by mutation from SM. NM cells neither glide individually nor cooperatively. FB(t), SM, and NM are indistinguishable with respect to fine structure, vegetative growth rate, glycerol-induced microcyst formation, spheroplasting, bacteriophage sensitivity, and responses to light. The motility mutants are more resistant to penicillin and more sensitive to actinomycin D than is the gliding wild type. The NM mutant is also a morphogenetic mutant; it is unable to form fruiting bodies.

MeSH Terms

Acridines/pharmacology Bacteria/cytology,drug effects,growth & development Cell Wall Culture Media Cycloserine/pharmacology Dactinomycin/pharmacology Genetics, Microbial Microscopy, Electron Microscopy, Phase-Contrast Movement Mutation Penicillin G/pharmacology Penicillin Resistance Protoplasts Sodium Chloride

Chemicals

Acridines Culture Media Dactinomycin Sodium Chloride Cycloserine Penicillin G

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Burchard R P

References (17)

17 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.104.2.940-947.1970

Published

1970-11-00

Pages

940-7

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC285078

Subset

External Links

PubMed source DOI: 10.1128/jb.104.2.940-947.1970 PMC Full Text View journal details

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Gliding motility mutants of Myxococcus xanthus.

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