Synthesis of vaccinia virus "early" and "late" messenger RNA in vitro with nucleoprotein structures isolated from infected cells.

Cell-Free System Centrifugation, Density Gradient Cytoplasm DNA Replication DNA, Viral/metabolism Escherichia coli/enzymology Genetics, Microbial Hybridization, Genetic In Vitro Techniques Magnesium/pharmacology Nucleoproteins/isolation & purification,metabolism Pancreas/enzymology RNA Nucleotidyltransferases/metabolism RNA, Messenger/biosynthesis,isolation & purification RNA, Viral/biosynthesis,isolation & purification Sucrose Time Factors Tritium Uridine/metabolism Vaccinia virus/growth & development,metabolism,pathogenicity Viral Proteins/biosynthesis

DNA, Viral Nucleoproteins RNA, Messenger RNA, Viral Viral Proteins Tritium Sucrose RNA Nucleotidyltransferases Magnesium Uridine