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PMID: 4920091 Published · ppublish English Journal Article

Mapping of a human genetic regulator element by somatic cell genetic analysis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 66 ·No. 4 ·1970-08-00 ·Pages 1220-7

Klebe RJ, Chen T, Ruddle RH

Abstract

The ES-2 esterase is a kidney-associated esterase and is expressed in a cloned mouse renal adenocarcinoma cell line. Somatic cell hybridization of the mouse renal adenocarcinoma with either mouse or human fibroblasts leads to the marked reduction of ES-2 esterase titer. The extinction of ES-2 esterase activity in mousexhuman hybrids is reversible. Extinction correlates with the presence of the human C(10) chromosome in the somatic cell hybrids; whereas reexpression of the ES-2 esterase is observed in hybrids which have lost C(10). Thus, the proposed regulator element involved is probably structurally linked to C(10); however, linkage to a member of the D or G groups cannot be completely excluded.

MeSH Terms
Adenocarcinoma Animals Cell Line Chromatography, Thin Layer Chromosomes, Human, 6-12 and X Esterases/metabolism Fibroblasts Genes, Regulator Humans Hybridization, Genetic In Vitro Techniques Karyotyping Kidney/enzymology Lung Mice Microscopy, Phase-Contrast
Chemicals
Esterases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Klebe R J
Chen T
Ruddle R H
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.66.4.1220
Published
1970-08-00
Pages
1220-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC335809
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.66.4.1220 PMC Full Text View journal details
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