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PMID: 4919990 Published · ppublish English Journal Article

Spermidine requirement for Bacillus thuringiensis ribosomes in cell-free phenylalanine incorporation.

Journal of bacteriology ·Vol. 103 ·No. 3 ·1970-09-00 ·Pages 734-40

Changchien LM, Aronson JN

Abstract

A cell-free system from Bacillus thuringiensis was found to actively incorporate phenylalanine into hot trichloroacetic acid-precipitable material in the presence of synthetic polynucleotide, ribosomes, S-100 supernatant, an energy-generating system, and guanosine triphosphate. Phenylalanine incorporation was absolutely dependent on the presence of spermidine in addition to magnesium ions, even when highly purified ribosomes were used. The spermidine effect could not be attributed to inhibition of nucleases. The ribosomal and supernatant fractions from Escherichia coli and B. thuringiensis could be substituted for each other in this system. The spermidine requirement was shown to be limited to the B. thuringiensis ribosome fraction.

MeSH Terms
Amines/metabolism Bacillus/metabolism Bacterial Proteins/biosynthesis Carbon Isotopes Cell-Free System Electrophoresis Escherichia coli Gels Ions Magnesium/pharmacology Peptide Biosynthesis Phenylalanine/metabolism Ribonucleases/metabolism Ribosomes/metabolism Temperature Trichloroacetic Acid
Chemicals
Amines Bacterial Proteins Carbon Isotopes Gels Ions Phenylalanine Trichloroacetic Acid Ribonucleases Magnesium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Changchien L M
Aronson J N
References (35)
35 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.103.3.734-740.1970
Published
1970-09-00
Pages
734-40
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC248151
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.103.3.734-740.1970 PMC Full Text View journal details
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