Effect of adenosine and deoxyadenosine on the incorporation and breakdown of thymidine in Escherichia coli K-12.

Journal of bacteriology ·Vol. 103 ·No. 2 ·1970-08-00 ·Pages 417-21

Yagil E, Rosner A

Abstract

Deoxyadenosine (AdR) and adenosine (AR) enhance the incorporation of thymidine (TdR) into bacterial deoxyribonucleic acid (DNA) by the inhibition of TdR phosphorolysis in vivo. Neither of the purine nucleosides has an effect on the reaction catalyzed by TdR phosphorylase in vitro. AdR induces TdR phosphorylase and both purine nucleosides induce purine nucleoside phosphorylase. AR can stimulate uptake of more TdR into bacterial DNA than AdR.

MeSH Terms

DNA, Bacterial/biosynthesis Enzyme Induction Escherichia coli/enzymology,metabolism Glucosyltransferases/metabolism Nucleosides/pharmacology Nucleotidyltransferases/metabolism Thymidine/metabolism Tritium

Chemicals

DNA, Bacterial Nucleosides Tritium Glucosyltransferases Nucleotidyltransferases Thymidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Yagil E

Rosner A

References (14)

14 references, click to expand

Mutant bacteria showing efficient utilization of thymidine.
J Bacteriol. 1966 Jun;91(6):2390-1 PMID: 5329290
Thymidine breakdown and thymine uptake in different mutants of Escherichia coli.
Biochim Biophys Acta. 1967 Jun 20;142(1):228-37 PMID: 4963399
Thymidine and thymine incorporation into deoxyribonucleic acid: inhibition and repression by uridine of thymidine phosphorylase of Escherichia coli.
J Bacteriol. 1967 Nov;94(5):1546-50 PMID: 4862197
The effect of nucleosides on the phosphorolysis of thymidine by normal and phage-infected cells of E. coli.
Biochem Biophys Res Commun. 1968 Jan 25;30(2):153-8 PMID: 4868091
Characteristics of the deo operon: role in thymine utilization and sensitivity to deoxyribonucleosides.
J Bacteriol. 1968 Aug;96(2):501-14 PMID: 4877128
A map of four genes specifying enzymes involved in catabolism of nucleosides and deoxynucleosides in Escherichia coli.
Mol Gen Genet. 1969 Aug 15;104(4):351-9 PMID: 4904508
Specificity and efficiency of thymidine incorporation in Escherichia coli lacking thymidine phosphorylase.
J Bacteriol. 1969 Sep;99(3):681-7 PMID: 4905532
Incorporation of 5-bromodeoxyuridine into DNA of wild type Escherichia coli and its use for the enrichment of auxotrophic mutants.
Mol Gen Genet. 1970;106(3):254-62 PMID: 4249283
Enzymes of the human erythrocyte. I. Purine nucleoside phosphorylase; isolation procedure.
J Biol Chem. 1957 Feb;224(2):879-87 PMID: 13405917
Limited thymidine uptake in Escherichia coli due to an inducible thymidine phosphorylase.
Biochim Biophys Acta. 1961 Apr 29;49:222-5 PMID: 13738908
Purification, kinetics, and repression control of bacterial trans-N-deoxyribosylase.
J Biol Chem. 1963 Feb;238:702-9 PMID: 13970089
SUBSTRATE SPECIFICITY AND INDUCTION OF THYMIDINE PHOSPHORYLASE IN ESCHERICHIA COLI.
J Biol Chem. 1964 Jun;239:1789-93 PMID: 14213352
UPTAKE AND INCORPORATION OF THYMINE, THYMIDINE, URACIL, URIDINE, AND 5-FLUOROURACIL INTO THE NUCLEIC ACIDS OF BACILLUS SUBTILIS.
J Bacteriol. 1965 Apr;89:1011-4 PMID: 14276087
Metabolism of adenosine and deoxyadenosine by growing cultures of Escherichia coli.
J Biol Chem. 1960 Feb;235:450-6 PMID: 14420732

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.103.2.417-421.1970

Published

1970-08-00

Pages

417-21

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC248097

Subset

External Links

PubMed source DOI: 10.1128/jb.103.2.417-421.1970 PMC Full Text View journal details

Analysis Services

Effect of adenosine and deoxyadenosine on the incorporation and breakdown of thymidine in Escherichia coli K-12.

Contact

WeChat Official Account