Segregation of functional sex factor into minicells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 66 ·No. 3 ·1970-07-00 ·Pages 815-22

Kass LR, Yarmolinsky MB

Abstract

The segregation of a bacterial plasmid, the sex factor F', has been investigated in a cell-division mutant of Escherichia coli which produces small anucleate cells (minicells). Significant amounts of isotopically labeled DNA segregate into minicells dependent upon the presence of F'. Minicells containing F'Gal or F'(lambda) are shown to donate the plasmid in conjugation. These results demonstrate that the sex factor may be dissociated from the bacterial chromosome and that this separation does not prevent its subsequent transfer.

MeSH Terms

Carbon Isotopes Cell Nucleus Centrifugation, Density Gradient Chromosomes, Bacterial Conjugation, Genetic DNA, Bacterial/metabolism Escherichia coli/cytology Mutation Sex Factors Thymidine/metabolism Tritium

Chemicals

Carbon Isotopes DNA, Bacterial Tritium Thymidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kass L R

Yarmolinsky M B

References (18)

18 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.66.3.815

Published

1970-07-00

Pages

815-22

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC283123

Subset

External Links

PubMed source DOI: 10.1073/pnas.66.3.815 PMC Full Text View journal details

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Segregation of functional sex factor into minicells.

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