A spectrophotometric study of the secondary structure of ribonucleic acid isolated from the smaller and larger ribosomal subparticles of rabbit reticulocytes.

The Biochemical journal ·Vol. 117 ·No. 1 ·1970-03-00 ·Pages 101-18

Cox RA

Abstract

The spectrum of RNA from the smaller and larger subparticles of rabbit reticulocyte ribosomes was studied as a function of pH, ionic strength, urea concentration and temperature. It was inferred that both RNA species form short double-helical segments of not more than about 10 base-pairs in length. Not more than about 70% of the base residues may be located in double-helical segments. RNA from the larger subparticle is richer in guanine and cytosine residues and its secondary structure is the more stable. These conclusions are based on the use of double-helical RNA from virus-like particles and of unfractionated Escherichia coli tRNA as model systems.

MeSH Terms

Animals Cytosine/analysis Escherichia coli Guanine/analysis Hydrogen-Ion Concentration Models, Biological RNA/analysis,isolation & purification RNA, Bacterial RNA, Viral Rabbits Reticulocytes/analysis Ribosomes/analysis Spectrophotometry Temperature Urea/analysis

Chemicals

RNA, Bacterial RNA, Viral Guanine RNA Cytosine Urea

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Cox R A

References (27)

27 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1170101

Published

1970-03-00

Pages

101-18

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1178836

Subset

External Links

PubMed source DOI: 10.1042/bj1170101 PMC Full Text View journal details

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A spectrophotometric study of the secondary structure of ribonucleic acid isolated from the smaller and larger ribosomal subparticles of rabbit reticulocytes.

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