Formation of all stable RNA species in Escherichia coli by posttranscriptional modification.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 65 ·No. 4 ·1970-04-00 ·Pages 1097-104

Pace B, Peterson RL, Pace NR

Abstract

The kinetics of accumulation of the known stable RNA species (23S, 16S, and 5S rRNA and tRNA) in Escherichia coli C122 were monitored by polyacrylamide gel electrophoresis of purified cellular RNA, following termination of brief pulse labeling with (32)P-orthophosphate. Isotopically labeled stable RNA species appear only after a time lag, while total cellular RNA and the ostensible precursors to the stable RNA classes accumulate from the earliest times examined. It is concluded that all the known stable RNA species in E. coli are the products of posttranscriptional modification.

MeSH Terms

Electrophoresis, Disc Escherichia coli/metabolism Genetic Code Kinetics Phosphates/metabolism Phosphorus Isotopes RNA, Bacterial/analysis,biosynthesis RNA, Transfer/biosynthesis Ribosomes/metabolism

Chemicals

Phosphates Phosphorus Isotopes RNA, Bacterial RNA, Transfer

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Pace B

Peterson R L

Pace N R

References (14)

14 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.65.4.1097

Published

1970-04-00

Pages

1097-104

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC283028

Subset

External Links

PubMed source DOI: 10.1073/pnas.65.4.1097 PMC Full Text View journal details

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Formation of all stable RNA species in Escherichia coli by posttranscriptional modification.

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