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PMID: 49060 Published · ppublish English Journal Article

A p60 polypeptide in the feline leukemia virus pseudotype of Moloney sarcoma virus with murine leukemia virus p30 antigenic determinants.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 6 ·1975-06-00 ·Pages 2380-4

Oskarsson MK, Robey WG, Harris CL, Fischinger PJ, Haapala DK, Vande Woude GF

Abstract

A 60,000-dalton polypeptide (p60) has been identified in the feline leukemia virus (FeLV) pseudotype of Moloney sarcoma virus [MSV(FeLV)]. This polypeptide is present in the purified virus complex in concentrations greater than either the murine p30 or the feline p27. Purified p60 crossreacts immunologically with murine p30 group antiserum and contains several interspecies determinants, whereas the group specific determinant of FeLV p27 is not detected. Comparison of peptide fingerprints of p60 and murine p30 show many peptides in common. Limited digestion of p60 with either trypsin or chymotrypsin produced p30-35 and p20 peptides which retain the MuLV p30 group and interspecies antigenic activities. The p30 produced by both enzymes comigrates in polyacrylamide gels with the murine p30 of MSV(FeLV), thus suggesting that p60 may be an uncleaved precursor to p30.

MeSH Terms
Chromatography, Gel Chymotrypsin Cross Reactions Electrophoresis, Polyacrylamide Gel Epitopes Guanidines Immunodiffusion Leukemia Virus, Feline/analysis,immunology Leukemia Virus, Murine/immunology Molecular Weight Moloney murine leukemia virus/analysis,immunology Peptides/analysis,isolation & purification Trypsin
Chemicals
Epitopes Guanidines Peptides Chymotrypsin Trypsin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Oskarsson M K
Robey W G
Harris C L
Fischinger P J
Haapala D K
Vande Woude G F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.6.2380
Published
1975-06-00
Pages
2380-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432762
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.6.2380 PMC Full Text View journal details
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