Improved medium for detecting deoxyribonuclease-producing bacteria.

Applied microbiology ·Vol. 18 ·No. 6 ·1969-12-00 ·Pages 991-3

Smith PB, Hancock GA, Rhoden DL

Abstract

Incorporation of methyl green dye into an agar medium containing deoxyribo-nucleic acid results in an improved medium for detecting deoxyribonuclease-producing bacteria. Use of the dye makes it unnecessary to use acid to demonstrate deoxyribonuclease activity, thus allowing subculture or reincubation of colonies. The improved medium is highly sensitive and can be used for primary isolation of Staphylococcus aureus or group A Streptococcus pyogenes.

MeSH Terms

Bacteria/enzymology Bacteriological Techniques Culture Media Deoxyribonucleases/metabolism Staphylococcus/isolation & purification Streptococcus pyogenes/isolation & purification

Chemicals

Culture Media Deoxyribonucleases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Smith P B

Hancock G A

Rhoden D L

References (7)

7 references, click to expand

Use of the deoxyribonuclease test as an aid in the differentiation of Paracolobactrum (Hafnia) from serratia.
J Bacteriol. 1966 Jan;91(1):467-8 PMID: 5903113
A new medium, salt mannitol plasma agar, for the isolation of Staphylococcus aureus.
Am J Clin Pathol. 1967 Jan;47(1):30-9 PMID: 6015984
Streptococcal anti-desoxyribonuclease B: microtechnique determination.
J Lab Clin Med. 1968 May;71(5):867-73 PMID: 5647687
Modification of deoxyribonuclease test medium for rapid identification of Serratia marcescens.
Am J Clin Pathol. 1969 Jun;51(6):711-6 PMID: 4890372
Detection of nuclease activity in semisolid and broth cultures.
Appl Microbiol. 1969 Aug;18(2):174-6 PMID: 4185235
The determination of desoxyribonuclease activity by methyl green; application to serum.
Arch Biochem. 1950 Nov;29(1):41-53 PMID: 14790793
Rapid method for determining the activity of microorganisms on nucleic acids.
J Bacteriol. 1957 Apr;73(4):590-1 PMID: 13428699

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.18.6.991-993.1969

Published

1969-12-00

Pages

991-3

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC378181

Subset

External Links

PubMed source DOI: 10.1128/am.18.6.991-993.1969 PMC Full Text

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