Circular deoxyribonucleic acid from Shigella dysenteriae Y6R.

Journal of bacteriology ·Vol. 100 ·No. 2 ·1969-11-00 ·Pages 803-8

Rush MG, Gordon CN, Warner RC

Abstract

Circular deoxyribonucleic acid was isolated from Shigella dysenteriae Y6R and was found to consist of six species having molecular weights of 10(6), 1.3 x 10(6), 2.6 x 10(6), 3.8 x 10(6), 20 x 10(6), and 24 x 10(6) daltons. These size classes were partially resolved by sucrose density gradient centrifugation. The minicircles (10(6) and 1.3 x 10(6)) were found to have a buoyant density in CsCl of 1.710 g/ml. The 3.8 x 10(6) dalton class had a density of 1.707 g/ml. The two largest species had a density of 1.702 g/ml. Two other strains, S. sonnei II and S. dysenteriae 60, also contained circular deoxyribonucleic acid.

MeSH Terms

Centrifugation, Density Gradient DNA, Bacterial/analysis,isolation & purification Microscopy, Electron Molecular Weight Phosphorus Isotopes Shigella dysenteriae/analysis Shigella sonnei/analysis Ultracentrifugation

Chemicals

DNA, Bacterial Phosphorus Isotopes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Rush M G

Gordon C N

Warner R C

References (15)

15 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.100.2.803-808.1969

Published

1969-11-00

Pages

803-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC250161

Subset

External Links

PubMed source DOI: 10.1128/jb.100.2.803-808.1969 PMC Full Text View journal details

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