Properties of a cell fraction that repairs damage to the cell division mechanism of Escherichia coli.

Journal of bacteriology ·Vol. 97 ·No. 2 ·1969-02-00 ·Pages 500-5

Fisher WD, Adler HI, Shull FW, Cohen A

Abstract

A factor in bacterial cell extracts which induces cell division in filaments of Escherichia coli produced by irradiation was found to be associated with a heat-labile particulate fraction which sediments at about 100S. Frozen or lyophilized samples of the cell extracts were stable for considerable periods of time. Fractions purified by centrifugation contained 2% of the total protein of the extract but no measurable ribonucleic acid or deoxyribonucleic acid. The extracts were inactivated by incubation with phospholipase A, lipases, and detergents, but not by incubation with selected nucleases and proteases.

MeSH Terms

Bacterial Proteins/analysis Cell Division Centrifugation, Density Gradient DNA, Bacterial/analysis Detergents/pharmacology Enzymes/pharmacology Escherichia coli/analysis,radiation effects RNA, Bacterial/analysis Radiation Genetics Ultrasonics

Chemicals

Bacterial Proteins DNA, Bacterial Detergents Enzymes RNA, Bacterial

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Fisher W D

Adler H I

Shull F W

Cohen A

References (18)

18 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.97.2.500-505.1969

Published

1969-02-00

Pages

500-5

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC249718

Subset

External Links

PubMed source DOI: 10.1128/jb.97.2.500-505.1969 PMC Full Text View journal details

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Properties of a cell fraction that repairs damage to the cell division mechanism of Escherichia coli.

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