Heterogeneity of DNA methylation in murine L5178Y lymphoblasts.

Experientia ·Vol. 35 ·No. 9 ·1979-09-15 ·Pages 1166-7

Sawecka J, Kornacka L, Malec J

Abstract

Comparison of the extent of methylation in mouse DNA fragments rendered MgCl2 soluble after mild DNase II digestion of nuclei, with different reassociation rate and nucleoli-bound, revealed the existence of 3 regions of the genome particularly 5-methylcytosine-rich: the sequences considered to be related to the transcriptionally active chromatin with the highest content of this base and fast reassociating, as well as nucleolar DNA with somewhat lower proportion of the methylated cytosines.

MeSH Terms

Animals Chromatin/analysis Cytosine/analogs & derivatives,analysis DNA, Neoplasm/analysis Leukemia L5178/analysis Leukemia, Experimental/analysis Lymphocytes/analysis Magnesium Methylation Mice Nucleic Acid Renaturation

Chemicals

Chromatin DNA, Neoplasm Cytosine Magnesium

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Sawecka J

Kornacka L

Malec J

References (10)

10 references, click to expand

Structure of transcriptionally-active chromatin subunits.
Nucleic Acids Res. 1977 Sep;4(9):3155-73 PMID: 909802
Synchronization of L5178Y cells by treatment with hydroxyurea.
Arch Immunol Ther Exp (Warsz). 1975;23(5):693-9 PMID: 1240752
[Intragenomic distribution of 5-methylcytosine in eukaryotic DNA].
Dokl Akad Nauk SSSR. 1974;219(4):1007-9 PMID: 4434839
The purine and pyrimidine composition of deoxypentose nucleic acids.
Biochem J. 1951 May;48(5):584-90 PMID: 14838906
Nuclear RNA of normal and leukaemic human leucocytes.
Haematologia (Budap). 1971;5(1):25-36 PMID: 5286993
Organization, transcription, and regulation in the animal genome.
Q Rev Biol. 1973 Dec;48(4):565-613 PMID: 4592421
The distribution of modified and non-modified C-G doublets in BHK-21 cell DNA.
FEBS Lett. 1978 Jul 1;91(1):69-73 PMID: 668912
Methylation of nucleosomal and nuclease sensitive DNA.
Nucleic Acids Res. 1977 Sep;4(9):3097-108 PMID: 909800
Studies on the intranuclear distribution and properties of mouse satellite DNA.
Biochim Biophys Acta. 1968 Jun 18;161(1):76-93 PMID: 5690801
Sequence composition of the template-active fraction of rat liver chromatin.
Biochemistry. 1976 Jun 1;15(11):2472-83 PMID: 179568

Article Info

Journal

Experientia

Abbr.

Experientia

ISSN

0014-4754

DOI

10.1007/BF01963264

Published

1979-09-15

Pages

1166-7

Language

English

Region

Switzerland

NLM ID

0376547

Subset

External Links

PubMed source DOI: 10.1007/BF01963264

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Heterogeneity of DNA methylation in murine L5178Y lymphoblasts.

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