Mutant strains of Escherichia coli K-12 unable to form ubiquinone.

Journal of bacteriology ·Vol. 95 ·No. 5 ·1968-05-00 ·Pages 1591-8

Cox GB, Gibson F, Pittard J

Abstract

A strain of Escherichia coli was isolated which was unable to form ubiquinone. This mutant was obtained by selecting strains unable to grow on malate as sole source of carbon. Such strains were further screened by examination of the quinone content of cells grown on a glucose medium. A mutant unable to form vitamin K was also isolated by this procedure. A genetic analysis of the ubiquinoneless strain showed that it possessed two mutations affecting ubiquinone biosynthesis.

MeSH Terms

Chromatography Chromosome Mapping Chromosomes, Bacterial Conjugation, Genetic Drug Resistance, Microbial Escherichia coli/drug effects,metabolism Genetics, Microbial Lipids/analysis Malates/metabolism Mutation Streptomycin/pharmacology Transduction, Genetic Ubiquinone/biosynthesis Vitamin K/biosynthesis

Chemicals

Lipids Malates Vitamin K Ubiquinone Streptomycin

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Cox G B

Gibson F

Pittard J

References (13)

13 references, click to expand

[The biosynthesis of beta-galactosidase (lactase) in Escherichia coli; the specificity of induction].
Biochim Biophys Acta. 1951 Nov;7(4):585-99 PMID: 14904456
The role of shikimic acid in the biosynthesis of vitamin K2.
Biochem J. 1966 Jul;100(1):1-6 PMID: 5337721
The relationship of 4-hydroxybenzoic acid to lysine and methionine formation in Escherichia coli.
Biochem J. 1967 Jun;103(3):709-13 PMID: 4860543
Isolation of a quinone from beef heart mitochondria.
Biochim Biophys Acta. 1957 Jul;25(1):220-1 PMID: 13445756
The use of a ubiquinone-deficient mutant in the study of malate oxidation in Escherichia coli.
Biochim Biophys Acta. 1968 Jan 15;153(1):1-12 PMID: 4170445
THE BIOSYNTHESIS OF THE BENZOQUINONE RING OF UBIQUINONE FROM P-HYDROXYBENZALDEHYDE AND P-HYDROXYBENZOIC ACID IN RAT KIDNEY, AZOTOBACTER VINELANDII, AND BAKER'S YEAST.
Proc Natl Acad Sci U S A. 1964 Mar;51:444-50 PMID: 14171457
THE EFFECT OF ACRIDINE DYES ON MATING TYPE FACTORS IN ESCHERICHIA COLI.
Proc Natl Acad Sci U S A. 1960 Jan;46(1):57-64 PMID: 16590598
Ubiquinone deficiency in an auxotroph of Escherichia coli requiring 4-hydroxybenzoic acid.
Biochem J. 1967 Jun;103(3):714-9 PMID: 4292836
Hybridization between Escherichia coli and Shigella.
J Bacteriol. 1957 Oct;74(4):461-76 PMID: 13475269
THE GENETIC MAP OF ESCHERICHIA COLI K-12.
Genetics. 1964 Oct;50:659-77 PMID: 14221874
Modification of the penicillin technique for the selection of auxotrophic bacteria.
J Bacteriol. 1953 Mar;65(3):348-53 PMID: 13034750
Ubiquinone and vitamin K in bacteria. 2. Intracellular distribution in Escherichia coli and Micrococcus lysodeikticus.
Biochem J. 1962 Dec;85:550-4 PMID: 13971217
2,3-Dihydroxybenzoate as a bacterial growth factor and its route of biosynthesis.
Biochim Biophys Acta. 1967 Jul 25;141(2):319-31 PMID: 4292725

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.95.5.1591-1598.1968

Published

1968-05-00

Pages

1591-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC252181

Subset

External Links

PubMed source DOI: 10.1128/jb.95.5.1591-1598.1968 PMC Full Text View journal details

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Mutant strains of Escherichia coli K-12 unable to form ubiquinone.

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