Thermal chromatography of DNA-DNA reactions.

Biophysical journal ·Vol. 7 ·No. 5 ·1967-09-00 ·Pages 567-84

Cowie DB, Szafranski P

Abstract

Genetic relationships among bacteriophages and bacteria have been investigated using an extension of the DNA-agar technique. Thermal elution profiles, each characteristic of a specific DNA-DNA reaction, have been obtained from reactions involving homologous and heterologous DNAs. A sizable portion of the DNA of lysogenic (or semilysogenic DNA) viruses has been shown to be homologous to the DNA of their bacterial hosts. These homologies are contained in numerous sequences throughout the viral DNAs. Furthermore, DNA homologies exist among most temperate phage DNAs as well as with their respective hosts.

MeSH Terms

Bacteriolysis Bacteriophages Chromatography DNA, Bacterial/analysis DNA, Viral/analysis Escherichia coli Hot Temperature Phosphorus Isotopes

Chemicals

DNA, Bacterial DNA, Viral Phosphorus Isotopes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Cowie D B

Szafranski P

References (11)

11 references, click to expand

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Article Info

Journal

Biophysical journal

Abbr.

Biophys J

ISSN

0006-3495

DOI

10.1016/S0006-3495(67)86607-4

Published

1967-09-00

Pages

567-84

Language

English

Region

United States

NLM ID

0370626

PMCID

PMC1368082

Subset

External Links

PubMed source DOI: 10.1016/S0006-3495(67)86607-4 PMC Full Text View journal details

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